CAJ | 학술논문

目的构建表达中国B亚型HIV-1流行株env基因的DNA及重组腺病毒载体疫苗,将其用于预防或治疗HIV感染.方法构建质粒DNA疫苗pVR-gp160及重组腺病毒载体疫苗rAdV-gp160.将这两种疫苗以不同的方式免疫BALB/c小鼠,分别采用ELISPOT方法和ELISA方法检测免疫小鼠中HIV-1 Gp120特异性细胞免疫反应及抗体反应.结果 DNA疫苗单独免疫及DNA疫苗初免/腺病毒疫苗加强免疫的联合免疫方案皆可诱导较高水平的Gp120特异性细胞免疫反应;而在体液免疫方面,各实验组产生的Gp120特异性抗体水平都较低.结论所构建的DNA疫苗及rAdV疫苗能有效表达Gp160蛋白,并可有效激活机体的细胞免疫反应.
목적 구건표체중국B아형HIV-1류행주env기인적DNA급중조선병독재체역묘,장기용우예방혹치료HIV감염.방법 구건질립DNA역묘pVR-gp160급중조선병독재체역묘rAdV-gp160.장저량충역묘이불동적방식면역BALB/c소서,분별채용ELISPOT방법 화ELISA방법 검측면역소서중HIV-1 Gp120특이성세포면역반응급항체반응.결과 DNA역묘단독면역급DNA역묘초면/선병독역묘가강면역적연합면역방안개가유도교고수평적Gp120특이성세포면역반응;이재체액면역방면,각실험조산생적Gp120특이성항체수평도교저.결론 소구건적DNA역묘급rAdV역묘능유효표체Gp160단백,병가유효격활궤체적세포면역반응.
Objective To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines. Methods The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively. Results DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups. Conclusion DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.