CAJ | 학술논문

目的了解和掌握准噶尔瓮地大沙鼠鼠疫自然疫源地鼠疫菌质粒谱类型.方法提取新疆地区不同鼠疫自然疫源地及青藏高原喜玛拉雅旱獭鼠疫自然疫源地、昆仑山喜马拉雅旱獭鼠疫自然疫源地和内蒙古长爪沙鼠鼠疫自然疫源地的39株鼠疫菌质粒DNA,采用琼脂糖凝胶电泳法对其进行质粒谱图型分析.结果在检测的26株准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫菌中,有23株质粒谱为6×106、45×106、65×106,3株为6×106、45×106和72×106.结论准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫菌质粒谱分为两个类型:一为6×106、45×106、65×106质粒谱型,是该疫源地的主要质粒谱型,与邻近的北天山灰旱獭.长尾黄鼠鼠疫自然疫源地及内蒙占长爪沙鼠鼠疫自然疫源地、昆仑山喜马拉雅旱獭鼠疫自然疫源地和青藏高原喜马拉雅旱獭鼠疫自然疫源地的鼠疫菌主要质粒谱型一致;二为6×106、45×106和72×106质粒谱型,为少数型,在我国系首次发现.
목적 료해화장악준갈이옹지대사서서역자연역원지서역균질립보류형.방법 제취신강지구불동서역자연역원지급청장고원희마랍아한달서역자연역원지、곤륜산희마랍아한달서역자연역원지화내몽고장조사서서역자연역원지적39주서역균질립DNA,채용경지당응효전영법대기진행질립보도형분석.결과 재검측적26주준갈이분지대사서서역자연역원지서역균중,유23주질립보위6×106、45×106、65×106,3주위6×106、45×106화72×106.결론 준갈이분지대사서서역자연역원지서역균질립보분위량개류형:일위6×106、45×106、65×106질립보형,시해역원지적주요질립보형,여린근적북천산회한달.장미황서서역자연역원지급내몽점장조사서서역자연역원지、곤륜산희마랍아한달서역자연역원지화청장고원희마랍아한달서역자연역원지적서역균주요질립보형일치;이위6×106、45×106화72×106질립보형,위소수형,재아국계수차발현.
Objective To investigate the type of plasmid map of Y. pestis in the R. opimas natural plague loci in Junggar Basin. Methods A total of 39 plasmid DNA of Y. pestis which were isolated from the natural plague loci of Junggar Basin, Tianshan Mountain, Kunlun Mountain, Qinghai-Tibet Plateau and In-ner Mongolia were extracted by the methods of Kado and Liu. The plasmid map was analyzed by the methods of agarose gel eleetrophoretogram. Results Two types of plasmid map were found in 26 Y. pestis which were isolated from Junggar Basin. Of them 23 were 6 × 106, 45 × 106 and 65 × 106 type of plasmid map, and 3 were 6 × 106, 45 × 106 and 72 × 106 type. Conclusion There are two types of plasmid map in the R. opi-mus natural plague loci in Junggar Basin. One type, which is the dominant type in this area, is 6 × 106, 45 × 106 and 65 × 106 type. This type is also similar to the dominant plasmid map type of the nature plague loci of Tianshan Mountain, Kunlun Mountain, Qinghai-Tibet Plateau and Inner Mongolia. The other type is 6 × 106, 45 × 106 and 72 × 106 type, and this type is new plasmid map type of Y. pestis in our country.