遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2006年
9期
800-807
,共8页
胡衍彪%潘增祥%许丹%徐银学%刘红林%黄瑞华%胡志刚
鬍衍彪%潘增祥%許丹%徐銀學%劉紅林%黃瑞華%鬍誌剛
호연표%반증상%허단%서은학%류홍림%황서화%호지강
猪%卵巢%生殖细胞数%基因表达
豬%卵巢%生殖細胞數%基因錶達
저%란소%생식세포수%기인표체
pigs%ovary%germ cell number%gene expression
采集三周龄杜洛克×梅山(DM,n=30)杂种母猪与PIC-长大(PLL,n=53)杂种母猪卵巢,测定其生殖细胞数目,比较2个猪群间生殖细胞数的差异,并对4个卵泡发育相关基因在2个猪群卵巢中的mRNA表达进行定量分析,研究了这些基因的表达与生殖细胞数之间的关系.结果表明:DM杂种猪生殖细胞数显著高于PLL杂种母猪(P＜0.01);DM猪和PLL仔猪卵巢重没有明显差异(P=0.269),两猪群内生殖细胞数和卵巢重的相关性均不显著(分别R=0.335,P=0.07;R=0.119,P=0.398);DM杂种猪ESR和IGF1R的mRNA表达量与其生殖细胞数存在相关(分别R=0.648,P＜0.05;R=0.757,P＜0.01),FSHR和INHBAmRNA的表达与其生殖细胞数无显著相关;PLL母猪ESR、FSHR和IGFlRmRNA的表达与其生殖细胞数有相关性(分别R=0.435,P＜0.01;R=0.438,P＜0.01;R=0.292,P＜0.05),INHBA mRNA的表达与其生殖细胞数无显著相关.
採集三週齡杜洛剋×梅山(DM,n=30)雜種母豬與PIC-長大(PLL,n=53)雜種母豬卵巢,測定其生殖細胞數目,比較2箇豬群間生殖細胞數的差異,併對4箇卵泡髮育相關基因在2箇豬群卵巢中的mRNA錶達進行定量分析,研究瞭這些基因的錶達與生殖細胞數之間的關繫.結果錶明:DM雜種豬生殖細胞數顯著高于PLL雜種母豬(P＜0.01);DM豬和PLL仔豬卵巢重沒有明顯差異(P=0.269),兩豬群內生殖細胞數和卵巢重的相關性均不顯著(分彆R=0.335,P=0.07;R=0.119,P=0.398);DM雜種豬ESR和IGF1R的mRNA錶達量與其生殖細胞數存在相關(分彆R=0.648,P＜0.05;R=0.757,P＜0.01),FSHR和INHBAmRNA的錶達與其生殖細胞數無顯著相關;PLL母豬ESR、FSHR和IGFlRmRNA的錶達與其生殖細胞數有相關性(分彆R=0.435,P＜0.01;R=0.438,P＜0.01;R=0.292,P＜0.05),INHBA mRNA的錶達與其生殖細胞數無顯著相關.
채집삼주령두락극×매산(DM,n=30)잡충모저여PIC-장대(PLL,n=53)잡충모저란소,측정기생식세포수목,비교2개저군간생식세포수적차이,병대4개란포발육상관기인재2개저군란소중적mRNA표체진행정량분석,연구료저사기인적표체여생식세포수지간적관계.결과표명:DM잡충저생식세포수현저고우PLL잡충모저(P＜0.01);DM저화PLL자저란소중몰유명현차이(P=0.269),량저군내생식세포수화란소중적상관성균불현저(분별R=0.335,P=0.07;R=0.119,P=0.398);DM잡충저ESR화IGF1R적mRNA표체량여기생식세포수존재상관(분별R=0.648,P＜0.05;R=0.757,P＜0.01),FSHR화INHBAmRNA적표체여기생식세포수무현저상관;PLL모저ESR、FSHR화IGFlRmRNA적표체여기생식세포수유상관성(분별R=0.435,P＜0.01;R=0.438,P＜0.01;R=0.292,P＜0.05),INHBA mRNA적표체여기생식세포수무현저상관.
In this study, the ovarian germ cell number was counted in 3-week-old Duroc × Meishan (DM, n=30) and PIC × (Landrace × Large White) (PLL, n=53) gilts, and the mRNA expression levels of four reproduction-related genes were investigated by quantitative RT-PCR. Correlation of germ cell number with the expression level of these genes was analyzed. Results showed that the germ cell number of DM was significantly higher than that of PLL gilts (P＜0.01), although there was no significant difference between the ovarian weight of DM and PLL gilts (P=0.269). No significant correlation existed between germ cell number and ovarian weight in the two gilt groups (R=0.335, P=0.07; R=0.119, P=0.398, respectively). A significant correlation was found between the germ cell number and expression level of ESR and IGF1R mRNA in DM gilts (R=0.648, P＜0.05; R=0.757, P＜0.01, respectively), but the correlation between the germ cell number and expression level of FSHR and INHBA mRNA did not reach statistical significance. Significant correlation was found between the germ cell number and the expression level of ESR, FSHR, and IGF1R mRNA in PLL gilts (R=0.435, P＜0.01; R=0.438, P＜0.01; R=0.292, P＜0.05, respectively), but not with INHBA mRNA in PLL gilts.
