CAJ | 학술논문

选用60头26日龄左右"杜×长×大"三元杂交断奶仔猪,按体重和性别随机分成3组,每组20头,分别饲喂基础日粮、基础日粮+3 000 mg·kg~(-1)氧化锌和基础日粮+500 mg·kg~(-1)蛋氨酸锌.试验期70 d.分别于断奶后0、7、14 、21、42、70 d经前腔静脉采血并收集粪便,用原子吸收光谱仪检测血清及粪便中铜、铁、锌水平.分别在试验的14 d和70 d,每组选5头仔猪放血致死,分别取心、肝、肾、脾、胸腺等组织,测定铜、铁、锌的含量.结果表明,仔猪断奶后,血清中铜、锌水平显著或极显著下降,而粪便铜、铁、锌含量显著或极显著升高;断奶后14 d,添加不同锌源的高锌日粮能显著提高断奶仔猪心、肝、肾、血清锌的含量,提高粪便中铜、锌的含量,显著或极显著降低仔猪血清铜、心和胸腺铜及脾铁水平;断奶后70 d,显著或极显著提高断奶仔猪心、肝、肾、脾、胸腺、血清锌的含量及提高肾、胸腺铁及粪便铜、铁、锌的含量,显著或极显著降低仔猪血清铜和心、脾、胸腺铜水平及降低血清铁和心、肝、脾铁含量.高锌日粮能增加断奶应激仔猪体内锌水平及粪便中铜、铁、锌的含量,降低血清及部分组织中铜、铁的含量.
선용60두26일령좌우"두×장×대"삼원잡교단내자저,안체중화성별수궤분성3조,매조20두,분별사위기출일량、기출일량+3 000 mg·kg~(-1)양화자화기출일량+500 mg·kg~(-1)단안산자.시험기70 d.분별우단내후0、7、14 、21、42、70 d경전강정맥채혈병수집분편,용원자흡수광보의검측혈청급분편중동、철、자수평.분별재시험적14 d화70 d,매조선5두자저방혈치사,분별취심、간、신、비、흉선등조직,측정동、철、자적함량.결과표명,자저단내후,혈청중동、자수평현저혹겁현저하강,이분편동、철、자함량현저혹겁현저승고;단내후14 d,첨가불동자원적고자일량능현저제고단내자저심、간、신、혈청자적함량,제고분편중동、자적함량,현저혹겁현저강저자저혈청동、심화흉선동급비철수평;단내후70 d,현저혹겁현저제고단내자저심、간、신、비、흉선、혈청자적함량급제고신、흉선철급분편동、철、자적함량,현저혹겁현저강저자저혈청동화심、비、흉선동수평급강저혈청철화심、간、비철함량.고자일량능증가단내응격자저체내자수평급분편중동、철、자적함량,강저혈청급부분조직중동、철적함량.
Sixty "Duroc xLandracexYorkshire" crossbred weanling piglets ( (26±2) day of age) were randomly divided into 3 groups of 20 piglets each by weight and sex, fed with basal diet, basal diet plus 3 000 mg· kg~(-1) zinc (from ZnO) , and basal diet plus 500 mg · kg~(-1) zinc (from Zn-Met) respectively. The trial was conducted for 70 d. Blood samples were taken from the anterior vena cava and dejecta was collected on 0, 7, 14, 21, 42, 70 d postweaned. Heart, liver, kidney, spleen and thymus tissues in 15 piglets (5 per group) were collected at 14 d and 70 d postweaned for later analysis of trace minerals. Copper, iron, zinc concentrations of serum, tissues and dejecta were measured with atomic absorption spectrophotometer. The results indicated that the levels of serum Cu and serum Zn decreased significantly postweaned, and the contents of Cu, Fe, Zn were increased significantly postweaned. The different dozes and kinds of high zinc diets increased significantly the contents of zinc in heart, liver, kidney and sera, and the contents of Cu and Zn in dejecta, and decreased significantly the contents of Cu in sera, heart, thymus and the contents of Fe in spleen of weanling piglets on day 14 postweaned. The contents of zinc in heart, liver, kidney, spleen, thymus and sera were improved significantly, enhanced significantly the contents of Fe in kidney and thymus tissue and the contents of Cu, Fe, Zn in dejecta, and the contents of copper in sera, heart, spleen and thymus, decreased significantly the contents of Fe in sera, heart, liver and spleen of weanling piglets feeding high level zinc diets on 70 d postweaned. The high level zinc diets increased the levels of zinc in weanling stress piglets, increased the contents of Cu, Fe, Zn in dejecta and reduced the copper and iron levels in sera and some tissues of piglets.