CAJ | 학술논문

为了揭示北方杂交粳稻亲本的遗传背景,利用微卫星(SSR)分子标记对北方杂交粳稻亲本及其高代后续材料进行了遗传差异鉴定和籼性程度分析.26对SSR引物共扩增出71个等位基因,每对引物平均等位基因数为2.73个.用非加权类平均法(UPGMA)进行聚类分析,供试材料在相似系数0.66处可分成5组,各组间材料的遗传差异相对较大;64个恢复系材料的籼性程度参数值(AD_i)的变幅为0.056～0.684;24个不育系材料AD_i的变幅为0.087～0.381.分析表明,强优势杂交粳稻组合双亲之间遗传距离应在0.30～0.60,恢复系的AD_i应在0.26～0.50,不育系的AD_i不应高于0.20,杂交组合的AD_i应在0.20～0.40,同时杂交粳稻组合优势受母本的遗传背景的影响较大,母本自身应具有较高的产量潜力.
위료게시북방잡교갱도친본적유전배경,이용미위성(SSR)분자표기대북방잡교갱도친본급기고대후속재료진행료유전차이감정화선성정도분석.26대SSR인물공확증출71개등위기인,매대인물평균등위기인수위2.73개.용비가권류평균법(UPGMA)진행취류분석,공시재료재상사계수0.66처가분성5조,각조간재료적유전차이상대교대;64개회복계재료적선성정도삼수치(AD_i)적변폭위0.056～0.684;24개불육계재료AD_i적변폭위0.087～0.381.분석표명,강우세잡교갱도조합쌍친지간유전거리응재0.30～0.60,회복계적AD_i응재0.26～0.50,불육계적AD_i불응고우0.20,잡교조합적AD_i응재0.20～0.40,동시잡교갱도조합우세수모본적유전배경적영향교대,모본자신응구유교고적산량잠력.
To reveal the genetic basis of parents of japonica hybrid rice in the Northern China, genetic differentiation and indica component analysis were conducted on the main parents of japonica hybrid rice and their succeeding materials by using simple sequence repeats(SSR) marker.The genomic DNAs of 96 rice materials were amplified with 26 primers, and a total of 71 alleles were detected, with 2.73 alleles per primer on average. The molecular phylogenetic tree built by UPMGA method showed that the rice materials were divided into 5 groups at the genetic similarity coefficient of 0.66.Relatively great genetic differentiation was found among groups.The extent of indica component value (AD_i) of the 64 restorer lines was from 0.056 to 0.684, and that of 24 sterile lines was from 0.087 to 0.381. It is suggested that the extent of indica component value (AD_i) of a hybrid rice combination with high heterosis should be from 0.20 to 0.40, that of its restorer line should be from 0.26 to 0.50, and that of its sterile line should be below 0.20, and the genetic distance of its parents should vary from 0.30 to 0.60. Besides, the female parent of a hybrid rice combination with high heterosis should have a high yield potential, because the yield of a hybrid rice combination was more affected by its sterile line than its restorer line.