CAJ | 학술논문

目的:研究雷帕霉素(rapamycin)对胆囊癌GBC-SD细胞生长和转移的影响,探讨雷帕霉素治疗胆囊癌的临床应用前景.方法:采用MTT法检测不同浓度(12.5、25、50 nmol/L)的雷帕霉素对胆囊癌细胞增殖的影响,以流式细胞术检测不同浓度的雷帕霉素对细胞凋亡和细胞周期的变化,Transwell小室检测雷帕霉素对细胞迁移能力的影响,利用Western blotting检测胆囊癌细胞中雷帕霉素哺乳动物靶标(mammalian target of rapamycin,mTOR)及其磷酸化p-mTOR水平.结果:雷帕霉素可显著抑制胆囊癌GBC-SD细胞中mTOR的磷酸化,但对mTOR表达无影响.雷帕霉素显著抑制胆囊癌细胞的生长,并呈剂量依赖性抑制(P<0.01).雷帕霉素可引起胆囊癌细胞周期G_1/S阻滞和细胞凋亡;可显著抑制胆囊癌细胞的转移(P<0.01).结论:雷帕霉素能显著抑制胆囊癌细胞生长及转移,其机制可能与抑制p-mTOR通路、诱导细胞周期阻滞和细胞凋亡有关.
목적:연구뢰파매소(rapamycin)대담낭암GBC-SD세포생장화전이적영향,탐토뢰파매소치료담낭암적림상응용전경.방법:채용MTT법검측불동농도(12.5、25、50 nmol/L)적뢰파매소대담낭암세포증식적영향,이류식세포술검측불동농도적뢰파매소대세포조망화세포주기적변화,Transwell소실검측뢰파매소대세포천이능력적영향,이용Western blotting검측담낭암세포중뢰파매소포유동물파표(mammalian target of rapamycin,mTOR)급기린산화p-mTOR수평.결과:뢰파매소가현저억제담낭암GBC-SD세포중mTOR적린산화,단대mTOR표체무영향.뢰파매소현저억제담낭암세포적생장,병정제량의뢰성억제(P<0.01).뢰파매소가인기담낭암세포주기G_1/S조체화세포조망;가현저억제담낭암세포적전이(P<0.01).결론:뢰파매소능현저억제담낭암세포생장급전이,기궤제가능여억제p-mTOR통로、유도세포주기조체화세포조망유관.
Objective:To investigate the effect of rapamycin on cell growth and migration of gallbladder cancer GBC-SD cells, and to discuss its potential in clinical therapy of gallbladder cancer. Methods: Proliferation of GBC-SD cells treated with different concentrations of rapamycin (12.5, 25, and 50 mmol/L) was examined by MTT assay. Cell cycle distribu-tion and apoptosis of GBC-SD cells treated with different concentrations of rapamycin were determined by flow cytometry. Migration ability of GBC-SD cells was assessed by Transwell assay. The expression of mTOR (mammalian target of rapam-ycin) and its phosphorylation in GBC-SD cells were examined by Western blotting assay. Results: Rapamycin significant-ly inhibited the phosphorylation of roTOR, but had no influence on the expression of roTOR in GBC-SD cells. Rapamycin significantly inhibited the growth of GBC-SD cells in a dose-dependent manner (P < 0.01). Raparnycin induced apoptosis of GBC-SD cells and arrested them at the G_1/S phase. Furthermore, rapamycin also significantly suppressed migration of GBC-SD cells as showed by Transwell assay (P < 0.01). Conclusion: Rapamycin can remarkably inhibit the growth and migration of gallbladder cancer cells, probably by inhibition of p-roTOR pathway, induction of apoptosis and cell cycle ar-rest of gallbladder cancer cells.