CAJ | 학술논문

目的研究高机械指数、高剂量微泡造影剂条件下超声造影对心肌毛细血管通透性的影响并观察其恢复正常的时间.方法将30只Wistar雄性大鼠随机分为5组.A～D组采用相同的超声造影条件,但各组伊文思蓝(EB)注射时间不同:分别为超声造影前10 s、造影结束即刻、造影结束后5 min、造影结束后20 nun给予EB;E组为对照组(假造影组),即5 nun超声照射结束后即刻再注射造影剂微泡及EB.所有动物均在EB注射后5 min处死.测定心脏EB渗出面积百分比及单位心肌内EB含量.结果 A、B、C组EB渗出面积明显高于对照组(P<0.05),D组渗出面积与对照组无差异(P>0.05);A、B组心肌EB含量明显高于对照组(P<0.05),C及D组心肌EB含量与对照组无差异(P>0.05).各组造影前后心率及心律失常数目无差异(P>0.05).结论高机械指数超声联合高剂量微泡造影剂可使心肌毛细血管通透性增加,其恢复时间在超声造影结束后20 min内.
목적 연구고궤계지수、고제량미포조영제조건하초성조영대심기모세혈관통투성적영향병관찰기회복정상적시간.방법 장30지Wistar웅성대서수궤분위5조.A～D조채용상동적초성조영조건,단각조이문사람(EB)주사시간불동:분별위초성조영전10 s、조영결속즉각、조영결속후5 min、조영결속후20 nun급여EB;E조위대조조(가조영조),즉5 nun초성조사결속후즉각재주사조영제미포급EB.소유동물균재EB주사후5 min처사.측정심장EB삼출면적백분비급단위심기내EB함량.결과 A、B、C조EB삼출면적명현고우대조조(P<0.05),D조삼출면적여대조조무차이(P>0.05);A、B조심기EB함량명현고우대조조(P<0.05),C급D조심기EB함량여대조조무차이(P>0.05).각조조영전후심솔급심률실상수목무차이(P>0.05).결론고궤계지수초성연합고제량미포조영제가사심기모세혈관통투성증가,기회복시간재초성조영결속후20 min내.
Objective To investigate the impact of high-dose microbubbles induced by high mechanical index myocardial contrast echocardiography (MCE) on vascular permeability and its recovery time in rats. Methods Thirty male Wistar rats were randomized into 4 MCE groups (groups A-D) and a control group. In the MCE groups, Evans blue was injected at 10 s before MCE (A), immediately after the end of MCE (B), and at 5 rain (C) and 20 min after the end of MCE (D). In the control group, the microbubbles and Evans blue were injected at the end of a 5-min ultrasound exposure. All the rats were sacrificed 5 min after Evans blue injection, and the content of Evans blue in the myocardium and the percentage of Evans blue leakage area were determined. Results The percentage of Evans blue leakage area in groups A, B and C were significantly higher than that in the control group (P<0.05), while the percentage was similar between group D and the control group (P>0.05). Evans blue contents in groups A and B were significantly higher than that in the control group (P<0.05), but groups C and D showed comparable contents with the control group E (P>0.05). No significant changes of the heart rates and premature beat number were observed during and after MCE in these groups (P>0.05). Conclusion High mechanical index MCE and a high contrast dose may induce increased mierovascular leakage in rats, and the vascular permeability can recover in 20 rain after MCE.