CAJ | 학술논문

目的分析急性水肿型胰腺炎(AEP)和急性坏死型胰腺炎(ANP)大鼠血清蛋白质表达的差异,寻找判断AP病情的血清指标.方法采用4％或5％牛磺胆酸钠逆行注入胆胰管法分别制备AEP和ANP大鼠模型.应用血清蛋白质谱弱阳离子交换芯片(WCX2),采用表面增强激光解析离子化飞行时间质谱(surface-enhanced laser desorption/ionization time-of-flight mass spectrometry,SELDI-TOFMS)技术分析AEP和ANP大鼠血清蛋白质谱的差异.结果 AEP和ANP大鼠血清蛋白质在质荷比为1000 ～50000分子量之间有38个蛋白质差异表达(P＜0.05),其中AEP大鼠血清中分子量为9500和9700的两种蛋白质表达明显高于ANP大鼠.结论用SELDI-TOF-MS技术能检测出AEP与ANP大鼠血清蛋白质的差异表达.分子量9500和9700蛋白质的表达下降可能是AEP向ANP转化的征兆.
목적 분석급성수종형이선염(AEP)화급성배사형이선염(ANP)대서혈청단백질표체적차이,심조판단AP병정적혈청지표.방법 채용4％혹5％우광담산납역행주입담이관법분별제비AEP화ANP대서모형.응용혈청단백질보약양리자교환심편(WCX2),채용표면증강격광해석리자화비행시간질보(surface-enhanced laser desorption/ionization time-of-flight mass spectrometry,SELDI-TOFMS)기술분석AEP화ANP대서혈청단백질보적차이.결과 AEP화ANP대서혈청단백질재질하비위1000 ～50000분자량지간유38개단백질차이표체(P＜0.05),기중AEP대서혈청중분자량위9500화9700적량충단백질표체명현고우ANP대서.결론 용SELDI-TOF-MS기술능검측출AEP여ANP대서혈청단백질적차이표체.분자량9500화9700단백질적표체하강가능시AEP향ANP전화적정조.
Objective To investigate the difference of the protein expression in serum of rats with acute edematous pancreatitis (AEP) and acute necrotizing pancreatitis (ANP),and to investigate serum marker for acute pancreatitis severity.Methods The model of AEP and ANP was induced by retrograde injection of 4％ or 5％ sodium taurocholate into the biliopancreatic duct.Weak cation exchange (WCX2) and surface enhanced laser desorption/ionization time of flight mass spectrometry ( SELDI TOF MS) was used to investigate the difference of the protein expression in serum of rats with AEP and ANP.Results Thirty-eight spectral peak clusters which had significantly different signal intensities between AEP and ANP sera at mass charge ratio between 1000-50 000(P ＜0.05) were detected.The peak clusters at 9500 and 9700 in the sera of AEP were higher than that in ANP rata.Conclusions Serum analysis with SELDI-TOF MS can detect the difference of the protein expression in rats with AEP and ANP.The decreased expression of the protein of molecular weight of 9500 and 9700 may be a signal of AEP transition into ANP.