CAJ | 학술논문

目的探讨紫外激光激发膀胱癌组织细胞荧光谱分析对改进膀胱癌光动力学诊断的意义.方法以不含细胞培养液和血卟啉单甲醚混合液为对照,分别取浓度为0、10、100、200 mg/L的血卟啉单甲醚作为光敏剂,孵育膀胱癌组织细胞株T24 1～3 h,再以全固态紫外激光作为激发光源(波长355 nm)激发,检测膀胱癌组织细胞特征荧光光谱并分析药物峰位置.结果在可见光区域390～780 nm,不含细胞的培养液和血卟啉单甲醚混合液未出现特征峰;无血卟啉单甲醚光敏剂的膀胱癌组织细胞的自体峰在445～490 nm处;不同时间不同浓度血卟啉单甲醚光敏剂孵育的膀胱癌组织细胞除在445～490 nm处有自体峰外,在628.65 nm处均出现药物峰,200 mg/L血卟啉单甲醚孵育2 h时膀胱癌组织细胞药物峰及自体峰荧光强度达到最高,且药物峰的荧光强度超过了自体峰.结论紫外激光激发的膀胱癌组织细胞荧光谱性质稳定、强度佳,对改进膀胱癌光动力学诊断具有确切价值.
목적 탐토자외격광격발방광암조직세포형광보분석대개진방광암광동역학진단적의의.방법 이불함세포배양액화혈계람단갑미혼합액위대조,분별취농도위0、10、100、200 mg/L적혈계람단갑미작위광민제,부육방광암조직세포주T24 1～3 h,재이전고태자외격광작위격발광원(파장355 nm)격발,검측방광암조직세포특정형광광보병분석약물봉위치.결과 재가견광구역390～780 nm,불함세포적배양액화혈계람단갑미혼합액미출현특정봉;무혈계람단갑미광민제적방광암조직세포적자체봉재445～490 nm처;불동시간불동농도혈계람단갑미광민제부육적방광암조직세포제재445～490 nm처유자체봉외,재628.65 nm처균출현약물봉,200 mg/L혈계람단갑미부육2 h시방광암조직세포약물봉급자체봉형광강도체도최고,차약물봉적형광강도초과료자체봉.결론 자외격광격발적방광암조직세포형광보성질은정、강도가,대개진방광암광동역학진단구유학절개치.
Objective To explore the significance of fluorescence spectrum of bladder cancer tissue cells induced by ultraviolet light for improvement of photodynamics diagnosis in bladder cancer.Methods A cell-free mixture of culture medium and photosensitizer(Hematoporphyrin monomethyl ether,HMME)was used as the control.The cells line T24 of bladder cancer tissue were incubated with different concentrations of HMME(0,10 mg/L,100 mg/L and 200 mg/L)for 1 to 3 hours.Thereafter,the specific fluorescence spectrum and the drug peak position of bladder cancer were detected using a 355 nm full solidstate ultraviolet laser for excitation.Results The cell-free mixture of culture medium and HMME did not demonstrate any specific spectrum within the range of visible light(390-780 nm).An inherent peak was shown in samples of HMME-free(0 mg/L)bladder cancer cells at 445-490 nm,whereas a drug peak was shown constantly at 628.65 nm in HMME-treated bladder cancer tissues,regardless of incubation time and doses of HMME.Highest level of inherent and drug peaks were noted in cell samples subjected to incubation with 200 mg/L HMME for 2 hours,where the drug peak Was even higher than the inherent one.Conclusion Fluorescence spectrum of bladder cancer tissue cells induced by ultraviolet laser demonstrates stable property and high intensity,which can be definitely promising for the improvement of photodynamics diagnosis in bladder cancer.