CAJ | 학술논문

目的通过中药坐浴不同干预时段和持续时间对大鼠肛门水肿恢复的研究,为临床探讨最佳疗效的中药坐浴方法提供依据.方法采用注射角叉菜胶致大鼠肛门水肿模型,将96只大鼠分早期干预及晚期干预2批,每批随机分为6组:空白1组、2组;模型1组、2组;给药1组、2组;运用中药坐浴的方法,1组坐浴持续时间为10 min,2组坐浴持续时间为20 min,观察肛门水肿的恢复情况.结果中药坐浴早期干预在大鼠肛门水肿而积消退、干湿重比值、微循环变化、皮肤温度指标中与模型组比较差异均有统计学意义(P<0.05),且坐浴效果20 min组优于10 min组(P<0.01);中药坐浴晚期干预仅在局部微循环变化及干湿重比值指标中与模型组比较差异有统计学意义(P<0.05).病理组织学检查给药组在局部组织炎性细胞浸润、组织间隙疏松程度及血管充血方面均较模型组有所改善.结论中药坐浴早期干预对大鼠肛门水肿的恢复有明显的效果,能促进肛门水肿的消退,改善水肿部位微循环,降低水肿局部皮肤温度,缓解肿胀区域炎症充血症状,且多项指标显示,坐浴20 min的效果优于10 min.
목적 통과중약좌욕불동간예시단화지속시간대대서항문수종회복적연구,위림상탐토최가료효적중약좌욕방법제공의거.방법 채용주사각차채효치대서항문수종모형,장96지대서분조기간예급만기간예2비,매비수궤분위6조:공백1조、2조;모형1조、2조;급약1조、2조;운용중약좌욕적방법,1조좌욕지속시간위10 min,2조좌욕지속시간위20 min,관찰항문수종적회복정황.결과 중약좌욕조기간예재대서항문수종이적소퇴、간습중비치、미순배변화、피부온도지표중여모형조비교차이균유통계학의의(P<0.05),차좌욕효과20 min조우우10 min조(P<0.01);중약좌욕만기간예부재국부미순배변화급간습중비치지표중여모형조비교차이유통계학의의(P<0.05).병리조직학검사급약조재국부조직염성세포침윤、조직간극소송정도급혈관충혈방면균교모형조유소개선.결론 중약좌욕조기간예대대서항문수종적회복유명현적효과,능촉진항문수종적소퇴,개선수종부위미순배,강저수종국부피부온도,완해종창구역염증충혈증상,차다항지표현시,좌욕20 min적효과우우10 min.
Objective To investigate the effects of different time duration hip bathing with traditional Chinese medicine for rehabilitation of rats with anal edema, which can provide evidence for a best effective hip bathing method in the clinical application. Methods The rat model of anal edema was induced with carrageenan injection. Ninty six SD rats were divided into early intervene and latter intervene batches, every batches were randomly divided into six groups; normal control group 1, normal control group2, model matched groupl, model matched group2, hip bathing with TCM groupl, hip bathing with TCM group2. Hip bathing time duration were lOminutes and 20minutes. The rehabilitation of rats anal edema were observed. Results The comparison has been made among edema area, the ratio of dry/wet weight, microcirculation change, skin temperature, the difference is statistically significant in TCM early intervene hip bathing groups (P < 0. 05). There was a significant difference of edema proportion with 20 minutes duration between TCM hip bathing groups and model control groups (P < 0. 01 ). The comparison has been made among the ratio of dry/wet weight, microcirculation change, the difference is statistically significant in TCM latter intervene hip bathing groups(P< 0. 05). Histopathological examination showed local tissue inflammatory cell infiltration, the loosen degree of tissue space and capillary congestion of modle group were improved. Conclusions Hip bathing with traditional Chinese medicine in early intervene have obvious effect on rehabilitation of rats with anal edema, which can dispell anal edema, improving microcirculation could descend the temperature and promote the rehabilitation of anal edema, relieve inflammation and hyperemia. In the respect of edema proportion relieving, hip bathing with 20 minutes duration showed better effect than 10 minutes.