CAJ | 학술논문

目的探讨脂联素对气道平滑肌细胞凋亡的影响及相关机制的研究.方法体外培养大鼠气道平滑肌细胞株,逆转录-聚合酶链反应法检测气道平滑肌细胞脂联素受体的表达.将体外培养的大鼠气道平滑肌细胞于不间浓度的脂联素中孵育48 h,流式细胞仪测定细胞的凋亡率;Western blot法检测活性的一磷酸腺苷激活蛋白激酶(pho-AMPK)蛋白的表达.结果脂联素能够促进气道平滑肌细胞凋亡,并存在浓度依赖性(r=0.952,P<0.01);Western blot显示随着脂联素浓度的升高pho-AMPK的表达增加(r=0.907,P<0.01).结论脂联素可能通过激活一磷酸腺苷激活蛋白激酶进一步激活凋亡信号通路诱导气道平滑肌细胞凋亡.
목적 탐토지련소대기도평활기세포조망적영향급상관궤제적연구.방법 체외배양대서기도평활기세포주,역전록-취합매련반응법검측기도평활기세포지련소수체적표체.장체외배양적대서기도평활기세포우불간농도적지련소중부육48 h,류식세포의측정세포적조망솔;Western blot법검측활성적일린산선감격활단백격매(pho-AMPK)단백적표체.결과 지련소능구촉진기도평활기세포조망,병존재농도의뢰성(r=0.952,P<0.01);Western blot현시수착지련소농도적승고pho-AMPK적표체증가(r=0.907,P<0.01).결론 지련소가능통과격활일린산선감격활단백격매진일보격활조망신호통로유도기도평활기세포조망.
Objective To investigate the effects of adiponectin on apoptosis of airway smooth muscle cells (ASMC) and the related mechanism. Methods ASMC of rats were cultured in vitro. The expression of adiponectin receptors in ASMC was detected by reverse transcription-polymerase chain reaction. The rat ASMC cultured was incubated with different concentrations of adiponectin for 48 hours. Apoptosis rate was detected by flow cytometry. The protein expression of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) was detected by Western blot. Results Adiponectin could promote apoptosis of ASMC, and there was concentration-dependent ( r =0.952, P <0. 01). Western blot indicated that the expression of phosphorylated AMPK increased with the incremental concentrations of adiponectin(r =0. 907, P <0. 01). Conclusions Adiponectin may further activate the apoptotic signaling pathway and induce the apoptosis of ASMC by activating AMPK.