中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
7期
393-397
,共5页
郝彦琴%张玲荣%陈杰%赵龙凤
郝彥琴%張玲榮%陳傑%趙龍鳳
학언금%장령영%진걸%조룡봉
硫代乙酰胺%内毒素血症%剂量效应关系,药物%疾病模型,动物%肝
硫代乙酰胺%內毒素血癥%劑量效應關繫,藥物%疾病模型,動物%肝
류대을선알%내독소혈증%제량효응관계,약물%질병모형,동물%간
Thioacetamide%Endotoxemia%Dose-response relationship,drug%Disease models,animal%Liver
目的 探讨不同剂量硫代乙酰胺(TAA)所制备的大鼠肠源性内毒素血症(IETM)模型的量效关系.方法 将40只大鼠分为4组,每组10只.TAA组分别以200、400、600mg/kg剂量的TAA灌胃,24h后相同剂量TAA重复灌胃一次,建立不同剂量TAA致大鼠IETM的动物模型;健康对照组以等体积0.9%氯化钠溶液灌胃.观察造模后24、48h大鼠死亡情况,48h后采集存活大鼠腹主动脉血,检测血浆内毒素、血清ALT和AST,观察肝组织病理变化.采用单因素方差分析,组间比较采用t检验.结果 造模48h后,健康对照组无大鼠死亡,200mg/kgTAA模型组死亡2只,400mg/kg TAA模型组死亡5只,600mg/kg TAA模型组死亡8只.200、400、600mg/kg TAA模型组大鼠血清ALT水平分别为(305.09±116.78)、(901.67±274.31)和(1454.84±473.49)U/L,明显高于健康对照组的(47.81±22.61)U/L(t=14.583、25.896、20.596,均P＜0.05);200、400、600mg/kg TAA模型组大鼠血清AST水平分别为(465.88±139.96)、(884.37±250.90)和(1889.23±159.67)U/L,明显高于健康对照组的(69.33±22.04)U/L(t=12.988、18.455、13.542,均P＜0.05);200、400、600mg/kg TAA模型组大鼠血浆内毒素水平分别为(0.436±0.110)、(0.550±0.095)和(0.620±0.057)EU/mL,明显高于健康对照组的(0.103±0.056)EU/mL(t=7.335、5.260、8.191,均P＜0.05).病理学显示不同剂量TAA模型组有不同程度的肝细胞变性坏死.结论 TAA剂量为200～600mg/kg时可成功制作IETM模型,200mg/kg TAA模型组大鼠死亡率较低,适于进一步的实验研究.
目的 探討不同劑量硫代乙酰胺(TAA)所製備的大鼠腸源性內毒素血癥(IETM)模型的量效關繫.方法 將40隻大鼠分為4組,每組10隻.TAA組分彆以200、400、600mg/kg劑量的TAA灌胃,24h後相同劑量TAA重複灌胃一次,建立不同劑量TAA緻大鼠IETM的動物模型;健康對照組以等體積0.9%氯化鈉溶液灌胃.觀察造模後24、48h大鼠死亡情況,48h後採集存活大鼠腹主動脈血,檢測血漿內毒素、血清ALT和AST,觀察肝組織病理變化.採用單因素方差分析,組間比較採用t檢驗.結果 造模48h後,健康對照組無大鼠死亡,200mg/kgTAA模型組死亡2隻,400mg/kg TAA模型組死亡5隻,600mg/kg TAA模型組死亡8隻.200、400、600mg/kg TAA模型組大鼠血清ALT水平分彆為(305.09±116.78)、(901.67±274.31)和(1454.84±473.49)U/L,明顯高于健康對照組的(47.81±22.61)U/L(t=14.583、25.896、20.596,均P＜0.05);200、400、600mg/kg TAA模型組大鼠血清AST水平分彆為(465.88±139.96)、(884.37±250.90)和(1889.23±159.67)U/L,明顯高于健康對照組的(69.33±22.04)U/L(t=12.988、18.455、13.542,均P＜0.05);200、400、600mg/kg TAA模型組大鼠血漿內毒素水平分彆為(0.436±0.110)、(0.550±0.095)和(0.620±0.057)EU/mL,明顯高于健康對照組的(0.103±0.056)EU/mL(t=7.335、5.260、8.191,均P＜0.05).病理學顯示不同劑量TAA模型組有不同程度的肝細胞變性壞死.結論 TAA劑量為200～600mg/kg時可成功製作IETM模型,200mg/kg TAA模型組大鼠死亡率較低,適于進一步的實驗研究.
목적 탐토불동제량류대을선알(TAA)소제비적대서장원성내독소혈증(IETM)모형적량효관계.방법 장40지대서분위4조,매조10지.TAA조분별이200、400、600mg/kg제량적TAA관위,24h후상동제량TAA중복관위일차,건립불동제량TAA치대서IETM적동물모형;건강대조조이등체적0.9%록화납용액관위.관찰조모후24、48h대서사망정황,48h후채집존활대서복주동맥혈,검측혈장내독소、혈청ALT화AST,관찰간조직병리변화.채용단인소방차분석,조간비교채용t검험.결과 조모48h후,건강대조조무대서사망,200mg/kgTAA모형조사망2지,400mg/kg TAA모형조사망5지,600mg/kg TAA모형조사망8지.200、400、600mg/kg TAA모형조대서혈청ALT수평분별위(305.09±116.78)、(901.67±274.31)화(1454.84±473.49)U/L,명현고우건강대조조적(47.81±22.61)U/L(t=14.583、25.896、20.596,균P＜0.05);200、400、600mg/kg TAA모형조대서혈청AST수평분별위(465.88±139.96)、(884.37±250.90)화(1889.23±159.67)U/L,명현고우건강대조조적(69.33±22.04)U/L(t=12.988、18.455、13.542,균P＜0.05);200、400、600mg/kg TAA모형조대서혈장내독소수평분별위(0.436±0.110)、(0.550±0.095)화(0.620±0.057)EU/mL,명현고우건강대조조적(0.103±0.056)EU/mL(t=7.335、5.260、8.191,균P＜0.05).병이학현시불동제량TAA모형조유불동정도적간세포변성배사.결론 TAA제량위200～600mg/kg시가성공제작IETM모형,200mg/kg TAA모형조대서사망솔교저,괄우진일보적실험연구.
Objective To investigate the correlation between dose and effect of thioacetamide (TAA) on rat model of intestinal endotoxemia. Methods The models of intestinal endotoxemia were induced by three different doses of TAA by gavage administration of TAA 200, 400, 600mg/kg respectively once per day for two days.The doses were given at same time point every day. Each group included 10 rats. The rats in the control group were administrated with 2 mL 0.9% NaCl saline gavage. The death of the rats was observed at 24 hours and 48 hours after administration. The blood samples of the living rats were drawn from abdominal aorta for determining the plasma endotoxin levels, serum alanine aminotransferase(ALT)and aspartated transaminase (AST) levels. The histopathological changes of liver were examined. Single factor analysis of variance was performed and comparision between groups was done using t test. Results No rat in the control group died. Two rats of 200 mg/kg TAA group, five rats of 400 mg/kg TAA group and eight rats of 600 mg/kg TAA group died during the experiment. The mean serum ALT levels of TAA model groups [(305.09±116.78)U/L,(901.67±274.31)U/L,(1454.84±473.49)U/L] were all significantly higher than that of the control group(47.81±22.61)U/L(t=14.583, 25.896 and 20.596, respectively; all P＜0.05). The mean serum AST levels of TAA model groups [(465.88±139.96)U/L, (884.37±250.90)U/L,(1889.23±159.67)U/L] were all significantly higher than that of the control group (69.33±22.04)U/L(t=12.988,18. 455 and 13.542, respectively; all P＜0.05). The mean plasma endotoxin levels of TAA model groups [(0.436±0.110)EU/mL, (0.550±0.095) EU/mL, (0.620±0.057)EU/mL] were all significantly higher than that of the control group (0.103±0.056)EU/mL(t=7.335, 5.260 and 8.191, respectively; all P＜0.05). The histological results of TAA model groups showed hepatic cell degeneration and necrosis in different degrees. Conclusions TAA with 200-600mg/kg is proper to establish the rat model of intestinal endotoxemia. The death rate of rats in the 200mg/kg TAA group is lower than those of other model groups, which suggests that 200mg/kg TAA may be the best dosage for establishing rat model for further studies.
