中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
2期
109-114
,共6页
常海刚%姜晓丹%陈中灿%杨璐军%法志强%杜谋选
常海剛%薑曉丹%陳中燦%楊璐軍%法誌彊%杜謀選
상해강%강효단%진중찬%양로군%법지강%두모선
TAG-1%粉样前体蛋白胞内段%神经胶质瘤%细胞凋亡
TAG-1%粉樣前體蛋白胞內段%神經膠質瘤%細胞凋亡
TAG-1%분양전체단백포내단%신경효질류%세포조망
Transient axonal glycoprotein-1%APP intracellualr domain%Glioma%Apoptosis
目的 探讨TAG-1对U251细胞的生长活力和粉样前体蛋白胞内段(AICD)、p53和表皮生长因子受体(EGFR)基因表达变化的影响.方法 以MTT法检测不同浓度TAG-1(0、5、10、20μg/mL)对U251细胞活力影响;免疫荧光细胞化学法观察U251细胞β淀粉样前体蛋白(APP)的表达;TUNEL法检测TAG-1对U251细胞凋亡形态学变化的影响;Real-time PCR检测TAG-1对U251细胞AICD、p53和EGFR基因表达的调控.结果 TAG-1没有抑制U251细胞的生长,相反表现出一定的促生长效应;APP广泛表达于U251细胞膜;在TAG-1浓度为10μg/mL时,U251细胞形态学检测没有发现明显的凋亡细胞,但AICD、p53和EGFR基因表达增加.结论 TAG-1在胶质瘤的增殖中发挥重要作用,但未发现其能通过TAG-1/APP/ AICD/p53或TAG-1/APP/AICD/EGFR信号途径促进U251细胞凋亡.
目的 探討TAG-1對U251細胞的生長活力和粉樣前體蛋白胞內段(AICD)、p53和錶皮生長因子受體(EGFR)基因錶達變化的影響.方法 以MTT法檢測不同濃度TAG-1(0、5、10、20μg/mL)對U251細胞活力影響;免疫熒光細胞化學法觀察U251細胞β澱粉樣前體蛋白(APP)的錶達;TUNEL法檢測TAG-1對U251細胞凋亡形態學變化的影響;Real-time PCR檢測TAG-1對U251細胞AICD、p53和EGFR基因錶達的調控.結果 TAG-1沒有抑製U251細胞的生長,相反錶現齣一定的促生長效應;APP廣汎錶達于U251細胞膜;在TAG-1濃度為10μg/mL時,U251細胞形態學檢測沒有髮現明顯的凋亡細胞,但AICD、p53和EGFR基因錶達增加.結論 TAG-1在膠質瘤的增殖中髮揮重要作用,但未髮現其能通過TAG-1/APP/ AICD/p53或TAG-1/APP/AICD/EGFR信號途徑促進U251細胞凋亡.
목적 탐토TAG-1대U251세포적생장활력화분양전체단백포내단(AICD)、p53화표피생장인자수체(EGFR)기인표체변화적영향.방법 이MTT법검측불동농도TAG-1(0、5、10、20μg/mL)대U251세포활력영향;면역형광세포화학법관찰U251세포β정분양전체단백(APP)적표체;TUNEL법검측TAG-1대U251세포조망형태학변화적영향;Real-time PCR검측TAG-1대U251세포AICD、p53화EGFR기인표체적조공.결과 TAG-1몰유억제U251세포적생장,상반표현출일정적촉생장효응;APP엄범표체우U251세포막;재TAG-1농도위10μg/mL시,U251세포형태학검측몰유발현명현적조망세포,단AICD、p53화EGFR기인표체증가.결론 TAG-1재효질류적증식중발휘중요작용,단미발현기능통과TAG-1/APP/ AICD/p53혹TAG-1/APP/AICD/EGFR신호도경촉진U251세포조망.
Objective To investigate the effects of transient axonal glycoprotein-1 (TAG-1) on activity of U251 cells and expressions of AICD, p53 and EGFR genes in the cells. Methods The viability of U251 cells was tested by MTT assay at 48 h following the addition of various concentrations of TAG-1 (0, 5, 10 and 20 μg/mL). The expression ofamyloid precursor protein (ALP) was detected by immunofluorescent staining. The apoptotic cells were examined by TUNEL. Real-time PCR was employed to detect the influence of TAG-1 on the expressions of AICD, p53 and EGFR genes in U251 cells. Results TAG-1 did not play an inhibitory effect on the proliferation of the U251 cells. APP was abundantly expressed on membrane of the U251 cells. U251 cells did not show apoptotic cells but increased expressions of AICD, p53 and EGFR genes were noted when U251 cells were exposed at 10 μg/mL of TAG-1. Conclusion TAG-1 plays an important role in regulating the proliferation of glioma and may not induce the apoptosis of U251 cells through the signal pathway of TAG-1/APP/AICD/p53 or TAG- 1/APP/AICD/EGFR.
