CAJ | 학술논문

目的观察二甲双胍对人肝癌Hep-G2细胞增殖、凋亡及裸鼠皮下瘤生长的影响.方法噻唑蓝(MTT)比色法分别检测二甲双胍作用于细胞后其细胞活力及生长抑制率;流式细胞术检测二甲双胍作用后细胞周期时相、凋亡率;免疫印迹法(Western blot)检测凋亡相关蛋白的表达;将Hep-G2细胞接种于裸鼠,观察不同浓度二甲双胍对裸鼠皮下瘤生长的影响.结果 20mmol/L二甲双胍作用细胞48h时,细胞生长周期阻滞于G0/G1期,细胞凋亡率为(20.57±3.16)%;Western blot检测显示bcl-2、bcl-xl蛋白表达随着药物浓度的增加而减少,Bid蛋白表达随着药物浓度增加而增加.高剂量二甲双胍组及联合用药组裸鼠皮下瘤体积显著小于对照组,20d时两组抑瘤率分别为40.8%、72.8%.结论二甲双胍通过线粒体介导的凋亡通路途径来诱导人肝癌细胞发生凋亡,并抑制肝癌细胞裸鼠瘤生长.
목적 관찰이갑쌍고대인간암Hep-G2세포증식、조망급라서피하류생장적영향.방법 새서람(MTT)비색법분별검측이갑쌍고작용우세포후기세포활력급생장억제솔;류식세포술검측이갑쌍고작용후세포주기시상、조망솔;면역인적법(Western blot)검측조망상관단백적표체;장Hep-G2세포접충우라서,관찰불동농도이갑쌍고대라서피하류생장적영향.결과 20mmol/L이갑쌍고작용세포48h시,세포생장주기조체우G0/G1기,세포조망솔위(20.57±3.16)%;Western blot검측현시bcl-2、bcl-xl단백표체수착약물농도적증가이감소,Bid단백표체수착약물농도증가이증가.고제량이갑쌍고조급연합용약조라서피하류체적현저소우대조조,20d시량조억류솔분별위40.8%、72.8%.결론 이갑쌍고통과선립체개도적조망통로도경래유도인간암세포발생조망,병억제간암세포라서류생장.
Objective To investigate the effect of metformin on proliferation, apoptosis of Hep-G2 cells and tumor growth in nude mice. Methods Hep-G2 cells were treated with metformin at different concentrations, and cell viability was measured by using methyl thiazol tetrazolium (MTT) method. Cell cycle and apoptosis rate were assayed by flow cytometry. The expression of apoptosis-related protein were detected by Western blotting.Nude mice were transplanted with Hep-G2 cells, and tumor growth inhibition rate was detected. Results After Hep-G2 cells were treated with 20 mmol/L metformin for 48 h, the growth cycle was arrested in G0/G1 phase, the apoptosis rate was (20.57±3.16)%, the expression of bcl-2 and bcl-xl proteins was down-regulated after metformin treatment, while the Bid protein was significantly increased, tumor size in the high-dose metformin group, cisplatin combined with metformin group were significantly reduced as compared with control group, and the inhibition rates in the high-dose metformin group, cisplatin combined with metformin group was 40.8% and 72.8% respectively. ConclusionMetformin inhibits proliferation of Hep-G2 cells, and induces apoptosis in vitro and significantly inhibits the growth of hepatocellular carcinoma in nude mice.