CAJ | 학술논문

目的:阐明Ets-1是否参与了实验性糖尿病视网膜病变过程.方法:腹腔注射链脲左菌素(STZ)来诱导糖尿病.STZ注射后4 wk,分离各组视网膜内总RNA和总蛋白,分别进行Northern blot和Western blot分析.结果:实验性糖尿病视网膜内Ets-1在mRNA水平和蛋白水平均明显高于对照组(P＜0.001).结论:实验结果表明Ets-1参与了实验性糖尿病视网膜的病理过程.进一步研究需要阐明Ets-1和VEGF关系.
목적:천명Ets-1시부삼여료실험성당뇨병시망막병변과정.방법:복강주사련뇨좌균소(STZ)래유도당뇨병.STZ주사후4 wk,분리각조시망막내총RNA화총단백,분별진행Northern blot화Western blot분석.결과:실험성당뇨병시망막내Ets-1재mRNA수평화단백수평균명현고우대조조(P＜0.001).결론:실험결과표명Ets-1삼여료실험성당뇨병시망막적병리과정.진일보연구수요천명Ets-1화VEGF관계.
AIM: To determine the involvement of Ets-1 in the pathological progress of the experimental diabetic retina. METHODS: Diabetes was induced by intraperitoneal injection of STZ. Total RNA and Total proteins were isolated from retinas of experimental and control eyes at 4 weeks after STZ-injection and were assessed by Northern blot analysis and Western blot analysis, respectively.RESULTS: Expression of both Ets-1 mRNA and Ets-1 protein was significantly increased in the experimental diabetic rat's retina after STZ-injection compared with the control group (P＜0.001).CONCLUSION: Our results indicated that Ets-1 was involved in the pathological progress of experimental diabetic retina.Further studies should be conducted to focus on the relationship between Ets-1 and VEGF in the diabetic retina.