CAJ | 학술논문

将磁性微粒与抗体的偶联,通过优化偶联条件提高了偶联效率,制备了高灵敏度的磁微粒生物探针;采用3-(2-螺旋金刚烷)-4-甲氧基-4-(3-邻氧酰苯基)-1,2-二氧杂环丁烷(AMPPD)--碱性磷酸酶(ALP)化学发光体系,建立了化学发光磁酶免疫检测方法;对检测方法进行了优化和改进,提高了系统的灵敏度和检测速度,并对HCG样品进行相关检测.结果表明,HCG浓度在0.15～150 IU/L范围内,光强随HCG浓度增大而增加,两者之间线性关系良好,相关系数r为0.960;检出限可达0.15 IU/L; 相对标准偏差(RSD)小于5%; 检测总时间少于1 h.本方法可以应用于其它免疫分子的检测,在临床免疫检测方面具有广阔的应用前景.
장자성미립여항체적우련,통과우화우련조건제고료우련효솔,제비료고령민도적자미립생물탐침;채용3-(2-라선금강완)-4-갑양기-4-(3-린양선분기)-1,2-이양잡배정완(AMPPD)--감성린산매(ALP)화학발광체계,건립료화학발광자매면역검측방법;대검측방법진행료우화화개진,제고료계통적령민도화검측속도,병대HCG양품진행상관검측.결과표명,HCG농도재0.15～150 IU/L범위내,광강수HCG농도증대이증가,량자지간선성관계량호,상관계수r위0.960;검출한가체0.15 IU/L; 상대표준편차(RSD)소우5%; 검측총시간소우1 h.본방법가이응용우기타면역분자적검측,재림상면역검측방면구유엄활적응용전경.
A highly sensitive magnetic enzymE-linked chemiluminescent immunoassay method was developed for the detection of human chorionic gonadotropin(HCG). The monoclonal antibody was covalently coupled on the surface of carboxylated magnetic beads to generate magnetic-biotargeting; Alkaline phosphatase(ALP) was utilized as a labeled reagent of another monoclonal antibody, whereas 3-(2-spimadamantane) 4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) was utilized as the chemiluminescent substrate. Based on this concept, a highly sensitive chemiluminescent immunoassay method was established to test HCG. Then, several modifications were made to optimize the method, and the detection sensitivity and procedure were improved accordingly. The detection of the assay could be fulfilled within 60 min and the test result of HCG concentration was linear over the range of 0.15 150 IU/L with good relativity(r=0.960). The relative standard deviation(RSD) were below 5% and the sensitivity of this method was 0.15 IU/L. The proposed method with wide linear range, simple operation and fast detection showed good prospect in practical application on-site.