CAJ | 학술논문

目的探讨血浆凝血因子Ⅶ(FⅦ)基因多态性在宁夏回、汉族老年急性冠状动脉综合征(ACS)患者中的分布特征.方法选择冠心病患者664例分为回族冠心病组(336例)和汉族冠心病组(328例),其中回族ACS患者194例,汉族ACS患者175例;同期选择健康体检者750例分为回族对照组(400例)和汉族对照组(350例),采用候选基因及病例对照研究的方法,以聚合酶链反应-限制性片段长度多态性技术,行FⅦ基因的R353Q、-323 0/10bp和HVR4基因多态性分析,同时采用重组可溶性组织因子法测定血浆FⅦa水平.结果回、汉族冠心痛组患者的血浆FⅦa水平均显著高于同族对照组(P<0.01).R353Q基因型及等位基因频率分布在回族冠心病组与回族对照组差异有统计学意义(P<0.01),-323 0/10 bp基因型及等位基因频率分布在汉族冠心病组与汉族对照组差异有统计学意义(P<0.01).回族ACS患者Q/Q+R/Q基因型频率高于回族非ACS患者,差异有统计学意义(P<0.01).结论宁夏回、汉族人群中存在FⅦ基因多态性;Q等位基因可能是回族人群ACS发生的遗传保护因子,FⅦa水平受R353Q基因多态性影响.
목적 탐토혈장응혈인자Ⅶ(FⅦ)기인다태성재저하회、한족노년급성관상동맥종합정(ACS)환자중적분포특정.방법 선택관심병환자664례분위회족관심병조(336례)화한족관심병조(328례),기중회족ACS환자194례,한족ACS환자175례;동기선택건강체검자750례분위회족대조조(400례)화한족대조조(350례),채용후선기인급병례대조연구적방법,이취합매련반응-한제성편단장도다태성기술,행FⅦ기인적R353Q、-323 0/10bp화HVR4기인다태성분석,동시채용중조가용성조직인자법측정혈장FⅦa수평.결과 회、한족관심통조환자적혈장FⅦa수평균현저고우동족대조조(P<0.01).R353Q기인형급등위기인빈솔분포재회족관심병조여회족대조조차이유통계학의의(P<0.01),-323 0/10 bp기인형급등위기인빈솔분포재한족관심병조여한족대조조차이유통계학의의(P<0.01).회족ACS환자Q/Q+R/Q기인형빈솔고우회족비ACS환자,차이유통계학의의(P<0.01).결론 저하회、한족인군중존재FⅦ기인다태성;Q등위기인가능시회족인군ACS발생적유전보호인자,FⅦa수평수R353Q기인다태성영향.
Objective To investigate the characteristics of distribution of coagulation factor (FⅦ) gene polymorphism in Ningxia Hui and Han patients with ACS. Methods 664 patients with CHD in Ningxia proven by angiography(Hui 336, Hun 328) were compared respectively with 400 Hui and 350 Han healthy blood donors serving as the control group. Their plasma levels of FⅦ was detected using recombinant soluble tissue factor method. The FⅦ gene R353Q,-323 0/10 bp and HVR4 genotypes were identified by polymerase chain reaction amplified genomic DNA method. Results Plasma FⅦa levels were significantly higher in patients with CHD than in control group. The distribution of genotypes and allele frequencies of R353Q in the Hui patient and control groups displayed significant difference, and there was also difference between the sub-groups of ACS and non-ACS (P < 0. 05). The difference of the-323 0/10 bp existed between patients with CHD and controls in the Han. FⅦa level was significantly higher in RR genotype than in Q allele carriers in the Hui. Conclusion The FⅦ gene polymorphism is present in the Hui and Hun population in Ningxia. The Q allele may be a protective factor against CHD in the Hui,and plasma FⅦa level may be influenced by the R353Q polymorphism.