时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
12期
3072-3074
,共3页
刘胜贵%李军%陈富成%刘霞%邓乐
劉勝貴%李軍%陳富成%劉霞%鄧樂
류성귀%리군%진부성%류하%산악
羊耳菊%水提取物%抑菌作用%二倍稀释法%药敏纸片扩散法
羊耳菊%水提取物%抑菌作用%二倍稀釋法%藥敏紙片擴散法
양이국%수제취물%억균작용%이배희석법%약민지편확산법
Inula cappa DC%Aqueous extracts%Antimicrobial activities%Serial two-fold method%Disk-diffusion method
目的 探讨羊耳菊不同器官水提取物的体外抑菌活性.方法 以金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌等10种微生物为试验菌株,采用药敏纸片扩散法、试管二倍稀释法,对羊耳菊水提取物进行抑菌试验.结果 羊耳菊全株各器官都具有较强的抑菌作用,其中根对10种供试菌种都有抑制作用,抑制范围最广;茎对9种供试菌种有抑制作用,叶对8种供试菌种有抑制作用.羊耳菊各器官对金黄色葡萄球菌与铜绿假单胞菌的抑制作用最强.羊耳菊根、茎、叶对金黄色葡萄球菌的MIC值分别为15.63,62.5,62.5 mg/ml,而其抑菌圈直径分别为14.3,13.8,13.5 mm;羊耳菊根,茎,叶对铜绿假单胞菌的MIC值分别为15.63,31.25,62.5 mg/ml,其抑菌圈直径分别为15.8,15.5,14.0 mm.结论 羊耳菊水提取物体外试验证实了该民间药物具有抗菌活性,也表明了羊耳菊是一种有开发潜力的新抗菌药物资源.
目的 探討羊耳菊不同器官水提取物的體外抑菌活性.方法 以金黃色葡萄毬菌、銅綠假單胞菌、白色唸珠菌等10種微生物為試驗菌株,採用藥敏紙片擴散法、試管二倍稀釋法,對羊耳菊水提取物進行抑菌試驗.結果 羊耳菊全株各器官都具有較彊的抑菌作用,其中根對10種供試菌種都有抑製作用,抑製範圍最廣;莖對9種供試菌種有抑製作用,葉對8種供試菌種有抑製作用.羊耳菊各器官對金黃色葡萄毬菌與銅綠假單胞菌的抑製作用最彊.羊耳菊根、莖、葉對金黃色葡萄毬菌的MIC值分彆為15.63,62.5,62.5 mg/ml,而其抑菌圈直徑分彆為14.3,13.8,13.5 mm;羊耳菊根,莖,葉對銅綠假單胞菌的MIC值分彆為15.63,31.25,62.5 mg/ml,其抑菌圈直徑分彆為15.8,15.5,14.0 mm.結論 羊耳菊水提取物體外試驗證實瞭該民間藥物具有抗菌活性,也錶明瞭羊耳菊是一種有開髮潛力的新抗菌藥物資源.
목적 탐토양이국불동기관수제취물적체외억균활성.방법 이금황색포도구균、동록가단포균、백색념주균등10충미생물위시험균주,채용약민지편확산법、시관이배희석법,대양이국수제취물진행억균시험.결과 양이국전주각기관도구유교강적억균작용,기중근대10충공시균충도유억제작용,억제범위최엄;경대9충공시균충유억제작용,협대8충공시균충유억제작용.양이국각기관대금황색포도구균여동록가단포균적억제작용최강.양이국근、경、협대금황색포도구균적MIC치분별위15.63,62.5,62.5 mg/ml,이기억균권직경분별위14.3,13.8,13.5 mm;양이국근,경,협대동록가단포균적MIC치분별위15.63,31.25,62.5 mg/ml,기억균권직경분별위15.8,15.5,14.0 mm.결론 양이국수제취물체외시험증실료해민간약물구유항균활성,야표명료양이국시일충유개발잠력적신항균약물자원.
Objective To study the antibacterial activities of aqueous extracts from the different organs of Inula cappa DC.Methods By using a disk-diffusion and serial two-fold dilutions method, minimal inhibitory concentration (MIC) and inhibitory circle were determined in the plant extracts against 10 medically important bacterial strains.Results The roots of Inula Cappa showed a stronger and broader spectrum of antibacterial activity. The roots of Inula Cappa showed a stronger and broader spectrum of antibacterial activity. The stems and the leaves inhibited 9,8 of 10 strains respectively. The MICs of the root, stem and leaf of Inula Cappa against Staphylococcus aureus were 15.63,62.5,62.5 mg/ml, and the diameters of the inhibition zone are 14.3,13.8,13.5 mm;and the MICs of the root, stem and leaves against Pseudomonas aeruginosa are 15.63,31.25,62.5 mg/ml, and the diameters of the inhibition zone were 15.8,15.5,14.0 mm. The aqueous extracts of the three organs of Inula Cappa DC had the weakest activities against Escherichia coli, Proteus vulgaris and Salmonella paratyphos.Conclusion This in vitro study corroborated the antimicrobial activity of the selected plants used in folkloric medicine and also showed that Inula Cappa DC. can be potential sources of new antimicrobial agents.
