CAJ | 학술논문

[目的]研究细菌个体间通讯信号N-己酰高丝氨酸内酯(C_6-HSL)是否可以影响植物的生长和抗病能力.[方法]以马铃薯脱毒苗为试验材料,C_6-HSL为诱导剂研究了经不同浓度C_6-HSL处理后,马铃薯生长、抵抗胡萝卜软腐欧文氏菌浸染的情况,特别研究了C_6-HSL对植株标志性防御酶活性和H_2O_2含量的影响.[结果]不同浓度的C__6-HSL能明显抑制马铃薯的出根率和生根数,而对株高、茎节数及叶片大小没有影响;植物标志性防御酶类中POD和SOD的活性以及H_2O_2的产生量经C_6-HSL诱导后显著提高,而PAL和PPO的活性则没有明显变化;抗病试验中,经C_6-HSL诱导的马铃薯植株能有效抑制胡萝卜软腐欧文氏菌的侵染,发病率明显低于对照组.[结论]细菌AHL有可能成为一种新的植物抗病激活剂.
[목적]연구세균개체간통신신호N-기선고사안산내지(C_6-HSL)시부가이영향식물적생장화항병능력.[방법]이마령서탈독묘위시험재료,C_6-HSL위유도제연구료경불동농도C_6-HSL처리후,마령서생장、저항호라복연부구문씨균침염적정황,특별연구료C_6-HSL대식주표지성방어매활성화H_2O_2함량적영향.[결과]불동농도적C__6-HSL능명현억제마령서적출근솔화생근수,이대주고、경절수급협편대소몰유영향;식물표지성방어매류중POD화SOD적활성이급H_2O_2적산생량경C_6-HSL유도후현저제고,이PAL화PPO적활성칙몰유명현변화;항병시험중,경C_6-HSL유도적마령서식주능유효억제호라복연부구문씨균적침염,발병솔명현저우대조조.[결론]세균AHL유가능성위일충신적식물항병격활제.
[Objective] The aim was to study whether C_6-HSL can affect plant growth and disease resistance ability. [Method] With potato virus-free plantlets as test materials and C_6-HSL as revulsant, the potato's growth and the ability of resistance against Erwinia carotovora subp. carotovora were tested after treated with different concentration of C_6-HSL. The effect of C_6-HSL on plant landmark defense enzyme activities and H_2O_2 concentration in potato leaf were measured especially. [Result] The results revealed that different concentration of C_6-HSL could obviously inhibit the growth rate of root and the number of roots, but have no effects on plant height, number of nodes and leaf size. The POD and SOD activities and the concentration of H_2O_2 showed a significantly increased when the potato implants were pretreated with C_6-HSL, however, no effect of C_6-HSL pretreatment on PAL and PPO activities were found. The ability of resistance aginst Erwinia carotovora subp. carotovora was signifinantly improved after treated by C_6-HSL. Incidence was obviously lower than the control group. [Conclusion] Bacteria AHL could be used as a new kind of plant disease resistance activator.