中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2010年
10期
21-23
,共3页
哮喘%白细胞介素19%免疫球蛋白E%嗜酸性粒细胞
哮喘%白細胞介素19%免疫毬蛋白E%嗜痠性粒細胞
효천%백세포개소19%면역구단백E%기산성립세포
Asthma%Interleukin-19%Immunoglobulin E%Oxyphil cells
目的 探讨支气管哮喘(简称哮喘)患者外周血白细胞介素(IL)-19的动态变化及其临床意义.方法 收集45例哮喘患者[急性发作期(哮喘急性发作组)和缓解期(哮喘缓解组)]和45例健康体检者(对照组)的血清,采用酶联免疫吸附法(ELISA)检测外周血IL-19、IgE水平,血细胞分析仪计数外周血嗜酸性粒细胞(EOS).结果 哮喘急性发作组患者外周血IL-19、IgE水平及EOS绝对计数分别为(327.92±245.79)ng/L、(260.48±108.77)kU/L、(498.29±96.46)×106/L;哮喘缓解组患者分别为(165.54±114.76)ng/L、(164.33±59.08)kU/L、(224.67±86.28)×106/L;对照组分别为(49.67±40.48)ng/L、(68.12±25.70)kU/L、(31.84 ±28.27)×106/L.哮喘急性发作组和哮喘缓解组IL-19、IgE水平和EOS绝对计数均明显高于对照组,差异有统计学意义(P<0.05).哮喘急性发作组IL-19水平和EOS绝对计数高于哮喘缓解组,差异有统计学意义(P<0.05).而哮喘急性发作组IgE水平与哮喘缓解组比较,差异无统计学意义(P>0.05).IL-19水平与IgE水平及EOS绝对计数均呈正相关(r=0.826、0.738,P<0.05).结论 外周血IL-19在哮喘不同时期上升程度不同,IL-19与哮喘发病机制有关,动态观察有助于哮喘临床诊断和病情监测.
目的 探討支氣管哮喘(簡稱哮喘)患者外週血白細胞介素(IL)-19的動態變化及其臨床意義.方法 收集45例哮喘患者[急性髮作期(哮喘急性髮作組)和緩解期(哮喘緩解組)]和45例健康體檢者(對照組)的血清,採用酶聯免疫吸附法(ELISA)檢測外週血IL-19、IgE水平,血細胞分析儀計數外週血嗜痠性粒細胞(EOS).結果 哮喘急性髮作組患者外週血IL-19、IgE水平及EOS絕對計數分彆為(327.92±245.79)ng/L、(260.48±108.77)kU/L、(498.29±96.46)×106/L;哮喘緩解組患者分彆為(165.54±114.76)ng/L、(164.33±59.08)kU/L、(224.67±86.28)×106/L;對照組分彆為(49.67±40.48)ng/L、(68.12±25.70)kU/L、(31.84 ±28.27)×106/L.哮喘急性髮作組和哮喘緩解組IL-19、IgE水平和EOS絕對計數均明顯高于對照組,差異有統計學意義(P<0.05).哮喘急性髮作組IL-19水平和EOS絕對計數高于哮喘緩解組,差異有統計學意義(P<0.05).而哮喘急性髮作組IgE水平與哮喘緩解組比較,差異無統計學意義(P>0.05).IL-19水平與IgE水平及EOS絕對計數均呈正相關(r=0.826、0.738,P<0.05).結論 外週血IL-19在哮喘不同時期上升程度不同,IL-19與哮喘髮病機製有關,動態觀察有助于哮喘臨床診斷和病情鑑測.
목적 탐토지기관효천(간칭효천)환자외주혈백세포개소(IL)-19적동태변화급기림상의의.방법 수집45례효천환자[급성발작기(효천급성발작조)화완해기(효천완해조)]화45례건강체검자(대조조)적혈청,채용매련면역흡부법(ELISA)검측외주혈IL-19、IgE수평,혈세포분석의계수외주혈기산성립세포(EOS).결과 효천급성발작조환자외주혈IL-19、IgE수평급EOS절대계수분별위(327.92±245.79)ng/L、(260.48±108.77)kU/L、(498.29±96.46)×106/L;효천완해조환자분별위(165.54±114.76)ng/L、(164.33±59.08)kU/L、(224.67±86.28)×106/L;대조조분별위(49.67±40.48)ng/L、(68.12±25.70)kU/L、(31.84 ±28.27)×106/L.효천급성발작조화효천완해조IL-19、IgE수평화EOS절대계수균명현고우대조조,차이유통계학의의(P<0.05).효천급성발작조IL-19수평화EOS절대계수고우효천완해조,차이유통계학의의(P<0.05).이효천급성발작조IgE수평여효천완해조비교,차이무통계학의의(P>0.05).IL-19수평여IgE수평급EOS절대계수균정정상관(r=0.826、0.738,P<0.05).결론 외주혈IL-19재효천불동시기상승정도불동,IL-19여효천발병궤제유관,동태관찰유조우효천림상진단화병정감측.
Objective To study the level of interleukin(IL)-19 in peripheral blood of patients with asthma and investigate its importance.Methods Serum samples were obtained from 45 asthmatic patients (acute attack group and catabasis group) and 45 subjects (control group).The level of IL-19 and IgE in peripheral blood was respectively determined with ELJSA.The accurate number of eosinophile granulocyte (EOS) in peripheral blood was counted with cytoanalyze.Results The levels of IL-19,IgE and the quantity of EOS were (327.92 ± 245.79) ng/L,(260.48 ± 108.77) kU/L,(498.29 ± 96.46) x 107L in acute attack group,(165.54 ± 114.76) ng/L,(164.33 ± 59.08) kU/L,(224.67 ± 86.28) x 106/L in catabasis group,and (49.67 ±40.48) ng/L,(68.12 ± 25.70) kU/L,(31.84 ± 28.27) x 106/L in control group.Compared with control group,the levels of IL-19,IgE and the quantity of EOS in acute attack group and catabasis group were obvious higher than those in control group (P < 0.05).The level of IL-19 and the quantity of EOS in acute attack group were higher than those in catabasis group (P <0.05),but there was no significant difference in the level of IgE between acute attack group and catabasis group (P > 0.05).Furthermore,the results of correlation analysis showed that the level of IL-19 positively correlated with the level of IgE and the quantity of EOS respectively (r = 0.826 >0.738,P< 0.05).Conclusions The ascending degree of the IL-19 level is different during various periods of asthma.It may be inferred that IL-19 can play an important role in the pathogenesis of asthma,and observing the change of the IL-19 level dynamically is useful in diagnosing asthma and monitoring patient's condition.
