CAJ | 학술논문

目的探讨大肠癌患者外周血淋巴细胞表型CD_8、CD_(28)的变化及其临床意义.方法应用CD_8、CD_(28)单克隆抗体,采用流式细胞仪技术,对50例大肠癌患者(大肠癌组)CD_8、CD_(28)进行测定,并与30例非肿瘤患者(对照组)进行比较.结果大肠癌组CD_8的表达显著高于对照组[(32.24±8.38)%比(22.18±7.55)%](P<0.01);而CD_(28)、CD_8~+,CDf的表达低于对照组[(52.03±10.94)%比(60.60±7.98)%,12.18-I-4.28比16.38±4.94](P<0.01).大肠癌组Dukes分期D期患者CD_8~+/CD_(28)~+的表达明显低于Dukes分期B期患者(P<0.05),有淋巴结转移患者CD_(28)、CD_8~+/CD_(28)~+的表达低于无淋巴结转移患者(P<0.01),已浸润浆膜层患者CD_(28)、CD_8~+/CD_(28)~+的表达则低于未浸润浆膜层患者(P<0.01).结论大肠癌患者CD_8、CD_(28)的表达与肿瘤的生物学行为密切相关.观察淋巴细胞表型CD_8、CD_(28)变化,对大肠癌患者的免疫状态及预后监测具有一定的参考价值.
목적 탐토대장암환자외주혈림파세포표형CD_8、CD_(28)적변화급기림상의의.방법 응용CD_8、CD_(28)단극륭항체,채용류식세포의기술,대50례대장암환자(대장암조)CD_8、CD_(28)진행측정,병여30례비종류환자(대조조)진행비교.결과 대장암조CD_8적표체현저고우대조조[(32.24±8.38)%비(22.18±7.55)%](P<0.01);이CD_(28)、CD_8~+,CDf적표체저우대조조[(52.03±10.94)%비(60.60±7.98)%,12.18-I-4.28비16.38±4.94](P<0.01).대장암조Dukes분기D기환자CD_8~+/CD_(28)~+적표체명현저우Dukes분기B기환자(P<0.05),유림파결전이환자CD_(28)、CD_8~+/CD_(28)~+적표체저우무림파결전이환자(P<0.01),이침윤장막층환자CD_(28)、CD_8~+/CD_(28)~+적표체칙저우미침윤장막층환자(P<0.01).결론 대장암환자CD_8、CD_(28)적표체여종류적생물학행위밀절상관.관찰림파세포표형CD_8、CD_(28)변화,대대장암환자적면역상태급예후감측구유일정적삼고개치.
Objective To investigate the expressions of cell phenotypes CD_8 and CD_(28) of peripheral blood lymphocytes in patients with colorectal cancer. Method CD_8 and CD_(28) of cell phenotypes were measured by flow cytometry in 50 patients with colorectal cancer (cancer group) and 30 nontumorous patients (control group). Results The expression of CD_8 in cancer group was significantly higher than that in control group [(32.24±8.38)% vs (22.18±7.55)%](P < 0.01). CD_(28) and CD_8~+/CD_(28)~+ were much lower than those in control group [(52.03±10.94)% vs (60.60±7.98)%,12.18±4.28 vs 16.38±4.94](P<0.01). CD_8~+/CD_(28)~+ in Dukes D stage patients were significantly lower than that in Dukes B stage patients (P<0.05). CD_(28) and CD_8~+/CD_(28)~+ in lymph node metastasis patients were much lower than those in lymph node negative patients (P < 0.01). CD_(28) and CD_8~+/CD_(28)~+ in serosa involved patients were lower than those in no serosa involved patients (P < 0.01). Conclusions The expressions of cell phenotypes CD_8 and CD_(28) of peripheral blood lymphocytes in patients with colorectal cancer are closely related to biological characteristics of the tumor. The assays of cell phenotypes CD_8 and CD_(28) might be useful for evaluating the immunal statement and prognosis of patients with colorectal cancer.