CAJ | 학술논문

口腔黏膜渗出液快速诊断试剂与血清ELISA试剂检测不同人群HIV-1抗体的评价
구강점막삼출액쾌속진단시제여혈청ELISA시제검측불동인군HIV-1항체적평개
A comparison study on the detection of anti-human immunodeficiency virus type 1 (HTV-1) antibodies in different populations with a new rapid test using oral mucosal transudate samples versus enzyme linked immunosorbent assay (ELISA) using serum samples

万方数据

中华皮肤科杂志中華皮膚科雜誌 중화피부과잡지
Chinese Journal of Dermatology
2011年 5期 302-305 ,共4页

吴焱%王克荣%韩晶%赵红心%曾辉%徐克沂%刘彦春%闫会文%李兴旺%伦文辉

오염%왕극영%한정%조홍심%증휘%서극기%류언춘%염회문%리흥왕%륜문휘

HIV抗体%口腔黏膜%渗出液和滤出液%酶联免疫吸附测定 HIV抗體%口腔黏膜%滲齣液和濾齣液%酶聯免疫吸附測定
HIV항체%구강점막%삼출액화려출액%매련면역흡부측정
HIV antibodies%Mouth mucosa%Exudates and transudates%Enzyme-linked immunosorbent assay

目的探讨口腔黏膜渗出液(OMT)快速诊断试剂与酶联免疫吸附试验(ELISA)试剂检测不同人群HIV-1抗体的一致性.方法对已经过生物梅里埃ELISA试剂检测血清HIV-1抗体阳性并经蛋白印迹(WB)试验确诊的HIV感染者200例(HIV阳性组),和经过生物梅里埃ELISA试剂检测血清HIV-1抗体阴性的健康人群600例(HIV阴性组)采集OMT标本,使用OMT快速诊断试剂进行HIV-1抗体检测.同时评价口腔黏膜渗出液快速诊断试剂在实际应用中的影响因素.结果 HIV阳性组200例中198例OMT标本检测为阳性,其中192例(96%)检测线清楚,易做阳性结果判断.4例(2%)"模糊",2例(1%)"很模糊",需经专业人员判断;2例(1%)检测线"看不见",为阴性.HIV阴性组600例OMT标本检测HIV抗体全为阴性.结论口腔黏膜渗出液快速诊断试剂与生物梅里埃血清ELISA诊断试剂检测HIV-1抗体相比,在HIV阳性标本中检测一致性为99.0%,在HIV阴性标本中检测一致性为100%,总体一致性为99.75%.
목적탐토구강점막삼출액(OMT)쾌속진단시제여매련면역흡부시험(ELISA)시제검측불동인군HIV-1항체적일치성.방법대이경과생물매리애ELISA시제검측혈청HIV-1항체양성병경단백인적(WB)시험학진적HIV감염자200례(HIV양성조),화경과생물매리애ELISA시제검측혈청HIV-1항체음성적건강인군600례(HIV음성조)채집OMT표본,사용OMT쾌속진단시제진행HIV-1항체검측.동시평개구강점막삼출액쾌속진단시제재실제응용중적영향인소.결과 HIV양성조200례중198례OMT표본검측위양성,기중192례(96%)검측선청초,역주양성결과판단.4례(2%)"모호",2례(1%)"흔모호",수경전업인원판단;2례(1%)검측선"간불견",위음성.HIV음성조600례OMT표본검측HIV항체전위음성.결론구강점막삼출액쾌속진단시제여생물매리애혈청ELISA진단시제검측HIV-1항체상비,재HIV양성표본중검측일치성위99.0%,재HIV음성표본중검측일치성위100%,총체일치성위99.75%.
Objective To evaluate the consistence in the detection of antibodies against HIV-1 between a new rapid test using oral mucosal transudate (OMT) samples and ELISA using serum samples. Methods Two-hundred patients who were positive for anti-HIV-1 antibodies by serum ELISA and confirmed by Western blot to be infected with HIV, and 600 healthy human controls negative for anti-HIV-1 antibodies by serum ELISA, were eligible for this study. OMT samples were collected from these subjects and subjected to a rapid test for anti-HIV-1 antibodies. The factors influencing the performance of the rapid test were analyzed. Results Of the 200 OMT specimens from HIV-infected patients, 198 showed positive reaction, 2 showed negative reaction. Among the 198 positive reactions, 192 (96%) were "clear" and easy to make decisions, 4 (2%) were "faint", 2(1%) were "very faint" and required professionals to make decisions. The rapid test was negative in all the 600 OMT specimens from the control group. Conclusions The consistence in the detection of anti-HIV-1 antibodies between the OMT rapid test and serum ELISA was 99% in HIV-positive specimens, 100% in HIV-negative specimens, and 99.75% in all the specimens.