CAJ | 학술논문

目的观察BMSCs移植对老化血管性痴呆大鼠模型认知功能的改善情况.方法对皮下注射4周D-半乳糖(D-gal)的大鼠行双血管结扎术(2VO),术后24 h移植BMSCs入侧脑室下区(SVZ).6周后行水迷宫检测,比较各组大鼠认知功能改变情况,同时行免疫荧光组织化学染色,观察BMSCs向神经元转化情况.结果 D-gal加2VO大鼠在水迷宫中所行距离及所花时间均较对正常大鼠明显增加,差异有统计学意义(P＜0.05),提示D-gal加2VO大鼠认知功能有明显缺损,符合老化血管性痴呆大鼠特征.移植6周后BMSCs围绕侧脑室周边分布,有1%的细胞分化为胶质细胞,2%的细胞分化为神经元.同时观察到移植BMSCs 6周后大鼠在水迷宫中所行距离及所花时间较老化血管性痴呆大鼠明显减少,差异有统计学意义(P＜0.05),但泳动速度没有明显差异(P＞0.05).结论 D-gal皮下注射加2VO模型能导致明显的认知功能缺损,而BMSCs移植能显著改善这种认知功能损害,考虑与BMSCs能促进宿主脑内神经功能重塑有关.
목적 관찰BMSCs이식대노화혈관성치태대서모형인지공능적개선정황.방법 대피하주사4주D-반유당(D-gal)적대서행쌍혈관결찰술(2VO),술후24 h이식BMSCs입측뇌실하구(SVZ).6주후행수미궁검측,비교각조대서인지공능개변정황,동시행면역형광조직화학염색,관찰BMSCs향신경원전화정황.결과 D-gal가2VO대서재수미궁중소행거리급소화시간균교대정상대서명현증가,차이유통계학의의(P＜0.05),제시D-gal가2VO대서인지공능유명현결손,부합노화혈관성치태대서특정.이식6주후BMSCs위요측뇌실주변분포,유1%적세포분화위효질세포,2%적세포분화위신경원.동시관찰도이식BMSCs 6주후대서재수미궁중소행거리급소화시간교노화혈관성치태대서명현감소,차이유통계학의의(P＜0.05),단영동속도몰유명현차이(P＞0.05).결론 D-gal피하주사가2VO모형능도치명현적인지공능결손,이BMSCs이식능현저개선저충인지공능손해,고필여BMSCs능촉진숙주뇌내신경공능중소유관.
Objective To evaluate whether bone marrow stromal cells (BMSCs) transplantation can improve the cognitive function of aged rats with vascular dementia. Methods Thirty rats were equally ramdomized into normal control group and 4 treatment groups; the 4 treatment groups received subcutaneous injection of D-galactose (D-gal) for 4 weeks; and then, two-vessel occlusion (2VO) was performed in 3 of the treatment groups; and 24 h after 2VO, D-gal+2VO+saline group and D-gal+2VO+BMSCs group were subjected to stereotactic injection of normal saline and BMSCs into the subventricular zone (SVZ), respectively. The cognitive function was examined by Morris water maze test 6 weeks after stereotactic injection; immunofluorescence staining was employed to observe the transplantation ratio of BMSCs to neurons. Results Increased times and distances during Morris water maze in rats of the D-gal+2VO group, D-gal+2VO+saline group and D-gal+2VO+BMSCs group were noted as compared with those in the controls, indicating that the cognitive function of rats in these 3 groups was obviously impaired; these rats had the characteristics of having vascular dementia.Transplanted BMSCs in the D-gal+2VO+BMSCs group distributed around the lateral ventricles, and acquired the phenotypes of neurons (2%) and astrocyte (1%) 6 weeks after the transplantation. In addition, compared with that in rats of the D-gal+2VO group and D-gal+2VO+saline group, the cognitive dysfunction of rats in the D-gal+2VO+BMSCs group was improved (needing less time and swimming shorter distance, no difference in speed of swimming). Conclusion The D-gal injection plus 2VO can result in cognitive dysfunction of rats, and the engrafted BMSCs may exhibit the beneficial effect on cognitive function.Neural function remolding caused by interaction between BMSCs and host brain may be responsible for the function improvement