CAJ | 학술논문

目的探讨制作简便、效果良好的海人酸(KA)点燃致痫模型方法。方法立体定向操作下于岛叶、杏仁核和侧脑室局部注射海人酸1.0μl,观察致痫大鼠的行为学和脑电图改变,检测其病理学变化,比较其点燃特征。结果行为学、脑电图和病理学证实岛叶点燃组与传统致痫(杏仁核、侧脑室)点燃组比较,具有相同的致痫特征;同时,岛叶点燃组初次发作时间为(59.96±4.36)min,静止持续时间为(11.90±0.54)d,明显快于杏仁核组和侧脑室组(P＜0.05)。结论岛叶本身具备致痫潜能,大鼠岛叶海人酸致痫模型具有缩短实验周期、降低实验成本的优势。
목적 탐토제작간편、효과량호적해인산(KA)점연치간모형방법。방법 입체정향조작하우도협、행인핵화측뇌실국부주사해인산1.0μl,관찰치간대서적행위학화뇌전도개변,검측기병이학변화,비교기점연특정。결과 행위학、뇌전도화병이학증실도협점연조여전통치간(행인핵、측뇌실)점연조비교,구유상동적치간특정;동시,도협점연조초차발작시간위(59.96±4.36)min,정지지속시간위(11.90±0.54)d,명현쾌우행인핵조화측뇌실조(P＜0.05)。결론 도협본신구비치간잠능,대서도협해인산치간모형구유축단실험주기、강저실험성본적우세。
Objective To discussion the methods of simpler production and better effect in the kainic acid induced epilepsy model. Methods By stereotactic operation,injection the kainic acid 1.0 μl to the insular, the amygdala and the lateral ventricle of the rats, observing the changes of the behavior, the EEG and the pathology and comparing the ignition characteristic in epileptic rats. Results The change of the behavior, the EEG and the pathology in epileptic rats confirmed that the rats of injection the kainic acid to insular can be successfully established experimental animal models. Compared to the traditional position (injection the kainic acid to amygdala and lateral ventricle) ,the insular ignite group primary seizure time is (59. 96 ±4. 36) min,static duration is(11. 90 ±0. 54) d,significantly faster than the amygdala group and the lateral ventricle group ( P ＜ 0. 05 ). Conclusion The insular has the seizure potency, the kainic acid injection the rat' s insular has the advantages in shortened test cycle and reduced the cost of experiments.