分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2001年
2期
20-22
,共3页
薛建国%傅敏恭%汪敬武%熊及波%杨伟平
薛建國%傅敏恭%汪敬武%熊及波%楊偉平
설건국%부민공%왕경무%웅급파%양위평
光度法%酪氨酸%人尿
光度法%酪氨痠%人尿
광도법%락안산%인뇨
研究了硝酸介质酪氨酸与α-显色剂的显色反应体系。显色产物在520nm处有最大吸收，表观摩尔吸光系数为6.19×103L.mol-1.cm-1。酪氨酸含量在0.02～0.16μmol/mL范围内符合比尔定律，该法可用于人尿中酪氨酸的测定。
研究瞭硝痠介質酪氨痠與α-顯色劑的顯色反應體繫。顯色產物在520nm處有最大吸收，錶觀摩爾吸光繫數為6.19×103L.mol-1.cm-1。酪氨痠含量在0.02～0.16μmol/mL範圍內符閤比爾定律，該法可用于人尿中酪氨痠的測定。
연구료초산개질락안산여α-현색제적현색반응체계。현색산물재520nm처유최대흡수，표관마이흡광계수위6.19×103L.mol-1.cm-1。락안산함량재0.02～0.16μmol/mL범위내부합비이정률，해법가용우인뇨중락안산적측정。
A new method for determination of tyrosine based on reaction oftyrosine with α-reagent in nitric acid was proposed. The apparent molar absorptivity of the product is 6.19×103L/(mol.cm) at 520 nm. The Beer's law was obeyed in the range of 0.02～0.16 μmol/mL. This method was applied to the determination of tyrosine in human urine.
