长江大学学报B（自然科学版）
長江大學學報B（自然科學版）
장강대학학보B（자연과학판）
JOURNAL OF YANGTZE UNIVERSITY(NATURAL SCIENCE EDITION)
2006年
1期
171-174
,共4页
昆虫病原真菌%28SrDNA%PCR-RFLP%UPGMA
昆蟲病原真菌%28SrDNA%PCR-RFLP%UPGMA
곤충병원진균%28SrDNA%PCR-RFLP%UPGMA
entomopathogenic fungi%28S rRNA gene%PCR-RFLP%UPGMA
利用核糖体28S rRNA基因的RFLP对18个昆虫病原真菌(座壳孢、轮枝菌和白僵菌属)菌株进行了分析.用4种限制性内切酶对PCR产物进行了消化并采用UPGMA法对扩增产物的限制性图谱构建了亲缘图.结果表明,属间、种间的多态性高于同种不同菌株间的多态性,并可以清楚地将座壳孢属不同种区分开来,而且轮枝菌不同菌株间存在着遗传差异.轮枝菌与白僵菌间的亲缘关系比其与座壳孢近.
利用覈糖體28S rRNA基因的RFLP對18箇昆蟲病原真菌(座殼孢、輪枝菌和白僵菌屬)菌株進行瞭分析.用4種限製性內切酶對PCR產物進行瞭消化併採用UPGMA法對擴增產物的限製性圖譜構建瞭親緣圖.結果錶明,屬間、種間的多態性高于同種不同菌株間的多態性,併可以清楚地將座殼孢屬不同種區分開來,而且輪枝菌不同菌株間存在著遺傳差異.輪枝菌與白僵菌間的親緣關繫比其與座殼孢近.
이용핵당체28S rRNA기인적RFLP대18개곤충병원진균(좌각포、륜지균화백강균속)균주진행료분석.용4충한제성내절매대PCR산물진행료소화병채용UPGMA법대확증산물적한제성도보구건료친연도.결과표명,속간、충간적다태성고우동충불동균주간적다태성,병가이청초지장좌각포속불동충구분개래,이차륜지균불동균주간존재착유전차이.륜지균여백강균간적친연관계비기여좌각포근.
Eighteen isolates of entomopathogenic fungi, including Aschersonia, Lecanicillium and Beauveria were analyzed by RFLP(restriction fragment length polymorphism) analysis of a 28S rRNA gene fragment. The data obtained by four different endonucleases were used to construct phenograms based on the UPGMA algorithm.Polymorphisms were found between genera and species, with fewer among isolates within species. PCR-RFLP within the 28S rRNA gene was sufficient to distinguish between Aschersonia species. RFLP data showed genetic diversity in Lecanicillium isolates at an infraspecific level. The UPGMA analysis results indicated the closer taxonomic affinity of Lecanicillium to Beauveria compared to Aschersonia.
