CAJ | 학술논문

采用生物化学方法对红螯螯虾胚胎发育过程中主要生化成分(糖类、蛋白质及氨基酸、脂类及脂肪酸)进行了测定和分析,以期了解胚胎形态发生与营养需求的关系.结果显示:红螯螯虾胚胎发育过程中糖类总体含量很低.总蛋白与总氨基酸(TAA)的含量在胚胎发育过程中均呈下降趋势.在必需氨基酸(EAA)中亮氨酸(Leu)和精氨酸(Arg)的含量较高,而在非必需氨基酸中谷氨酸(Glu)和天冬氨酸(Asp)的含量较高.总脂在胚胎发育过程中的含量总体呈下降趋势,其中性脂和磷脂的主要脂肪酸均为C16:0、C18:0 、C18:1ω9和C18:3ω3.研究认为:在红螯螯虾胚胎发育过程中,糖类主要参与多种特异复合物的合成以及甲壳构建,这些特异复合物在细胞分化中起着重要的信号传递作用.蛋白质和脂类都是红螯螯虾胚胎发育过程中主要的结构和能源物质,蛋白质主要在早期被利用而脂类主要在晚期起作用.饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)(C16:0,C18:0,C16:1ω7 和 C18:1ω9)主要作为能源物质被消耗而高不饱和脂肪酸(PUFA)(20:5ω3和22:6ω3)在胚胎发育过程中对于构建细胞膜以及调节中枢神经系统的发育起重要作用.
채용생물화학방법대홍오오하배태발육과정중주요생화성분(당류、단백질급안기산、지류급지방산)진행료측정화분석,이기료해배태형태발생여영양수구적관계.결과현시:홍오오하배태발육과정중당류총체함량흔저.총단백여총안기산(TAA)적함량재배태발육과정중균정하강추세.재필수안기산(EAA)중량안산(Leu)화정안산(Arg)적함량교고,이재비필수안기산중곡안산(Glu)화천동안산(Asp)적함량교고.총지재배태발육과정중적함량총체정하강추세,기중성지화린지적주요지방산균위C16:0、C18:0 、C18:1ω9화C18:3ω3.연구인위:재홍오오하배태발육과정중,당류주요삼여다충특이복합물적합성이급갑각구건,저사특이복합물재세포분화중기착중요적신호전체작용.단백질화지류도시홍오오하배태발육과정중주요적결구화능원물질,단백질주요재조기피이용이지류주요재만기기작용.포화지방산(SFA)화단불포화지방산(MUFA)(C16:0,C18:0,C16:1ω7 화 C18:1ω9)주요작위능원물질피소모이고불포화지방산(PUFA)(20:5ω3화22:6ω3)재배태발육과정중대우구건세포막이급조절중추신경계통적발육기중요작용.
The objective of the present study was to investigate the biochemical changes during embryogenesis of Cherax quadricarinatus, in order to evaluate the nutritional requirements of embryos. The carbohydrate content remained a minor constituent of yolk on the whole. The total protein and the total amino acids (TAA) were all observed down-trend during embryonic development. In the essential amino acids (EAA), the content of leucine and arginine acid were relatively high, but in the non-essential amino acid (NEAA), the quantitatively most important were glutamine and aspartic acid. The content of total lipid decreased during embryonic development, and the predominant fatty acids of both neutral lipid and polar lipid were all C16:0, C18:0, C18:1ω9 and C18:3ω3. It's indicated that the carbohydrate played an important role in synthesizing many specific compounds to participate in signal transmission and to form carapace. The protein and lipid were all the dominating construction and energy substances; protein was primarily consumed in the early stages but lipid in the late stages. The saturated fatty acids (SFA) and the monounsaturated fatty acids (MUFA) (C16:0, C18:0, C16:1ω7 and C18:1ω9) were always used for energetic purpose and the polyunsaturated fatty acids (PUFA) (C20:5ω3 and C22:6ω3) were important as structural components of cell membranes and the central nervous system during the development.