安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
19期
8989-8991
,共3页
贲永光%李康%李坤平%张伟永
賁永光%李康%李坤平%張偉永
분영광%리강%리곤평%장위영
海金沙%黄酮%自由基%清除作用
海金沙%黃酮%自由基%清除作用
해금사%황동%자유기%청제작용
Lygodium japonicum(Thunb)Sw%Flavone%Free radicals%Scavenging
[目的]比较不同溶剂提取海金沙总黄酮对自由基活性清除能力的大小.[方法]应用DPPH 法、邻苯三酚自氧化法和Fenton反应分别研究了浓度95%乙醇、乙酸乙酯、丙酮、乙酸、氯仿、甲醇提取物清除自由基活性的能力.[结果]6种溶剂提取得到的海金沙提取物对DPPH·、OH·和O2-·均有一定程度的清除作用, 不同溶剂所得提取物对自由基的清除作用均有差别,其中浓度95%乙醇提取得到的海金沙提取物对3种自由基清除效果均最好.[结论]海金沙可作为抗氧化物质资源进行开发利用.
[目的]比較不同溶劑提取海金沙總黃酮對自由基活性清除能力的大小.[方法]應用DPPH 法、鄰苯三酚自氧化法和Fenton反應分彆研究瞭濃度95%乙醇、乙痠乙酯、丙酮、乙痠、氯倣、甲醇提取物清除自由基活性的能力.[結果]6種溶劑提取得到的海金沙提取物對DPPH·、OH·和O2-·均有一定程度的清除作用, 不同溶劑所得提取物對自由基的清除作用均有差彆,其中濃度95%乙醇提取得到的海金沙提取物對3種自由基清除效果均最好.[結論]海金沙可作為抗氧化物質資源進行開髮利用.
[목적]비교불동용제제취해금사총황동대자유기활성청제능력적대소.[방법]응용DPPH 법、린분삼분자양화법화Fenton반응분별연구료농도95%을순、을산을지、병동、을산、록방、갑순제취물청제자유기활성적능력.[결과]6충용제제취득도적해금사제취물대DPPH·、OH·화O2-·균유일정정도적청제작용, 불동용제소득제취물대자유기적청제작용균유차별,기중농도95%을순제취득도적해금사제취물대3충자유기청제효과균최호.[결론]해금사가작위항양화물질자원진행개발이용.
[Objective] The research aimed to compare the scavenging abilities of total flavone from Lygodium japonicum with different solvents to free radical activity.[Method] DPPH method, pyrogallol method and Fenton reaction were used to study the scavenging ability of extracts with 95% ethanol, ethyl acetate, acetone, acetic acid, chloroform, methanol to free radical activity.[Result] Six kinds of solvent extracts from L.japonicum had certain scavenging ability to DPPH·, OH · and O2-·.The scavenging ability of extracted with different solvents to free radicals were difference.The extracts from L.japonicum with 95% ethanol had the best scavenging effects on three kinds of free radicals.[Conclusion] L.japonicum can be used as antioxidants for the development and utilization of resources.