CAJ | 학술논문

目的探讨外周血雌二醇水平和CD4~+CD25~+Treg细胞在反复自然流产发病机制中的作用.方法采用电化学发光法检测30例反复自然流产患者,90例正常妊娠女性(早、中、晚孕女性各30例)以及30例正常非孕女性外周血雌二醇水平;三色流式细胞术检测上述研究对象外周血CD~+CD25~+Treg细胞数量.结果早、中、晚孕组外周血雌二醇水平均明显高于非妊娠对照组(t值分别为7.42,13.66,14.69;均P＜0.05),并且孕周越长雌二醇水平越高;反复自然流产组明显低于正常非妊娠组(t=4.11,P<0.05).早、中、晚孕组外周血CD~+CD25~+Treg细胞数均明显高于正常非妊娠组(t值分别为2.22、8.9、5.91,均P<0.05),中孕组明显高于早孕组(t=6.31,P<0.05),晚孕组略低于中孕组,但差异无统计学意义;反复自然流产组明显低于正常非妊娠组(t=8.07,P<0.05).结论外周血雌二醇水平和CD4~+CD25~+Treg细胞数量减低可能下调母胎免疫耐受功能,参与介导反复自然流产的发生.
목적 탐토외주혈자이순수평화CD4~+CD25~+Treg세포재반복자연유산발병궤제중적작용.방법 채용전화학발광법검측30례반복자연유산환자,90례정상임신녀성(조、중、만잉녀성각30례)이급30례정상비잉녀성외주혈자이순수평;삼색류식세포술검측상술연구대상외주혈CD~+CD25~+Treg세포수량.결과 조、중、만잉조외주혈자이순수평균명현고우비임신대조조(t치분별위7.42,13.66,14.69;균P＜0.05),병차잉주월장자이순수평월고;반복자연유산조명현저우정상비임신조(t=4.11,P<0.05).조、중、만잉조외주혈CD~+CD25~+Treg세포수균명현고우정상비임신조(t치분별위2.22、8.9、5.91,균P<0.05),중잉조명현고우조잉조(t=6.31,P<0.05),만잉조략저우중잉조,단차이무통계학의의;반복자연유산조명현저우정상비임신조(t=8.07,P<0.05).결론 외주혈자이순수평화CD4~+CD25~+Treg세포수량감저가능하조모태면역내수공능,삼여개도반복자연유산적발생.
Objective To investigate the role of peripheral blood oestradiol and CD4~+CD25~+Treg cells in the pathogenesis of recurrent spontaneous abortion(RSA).Methods Electrochemiluminascence assay was adopted to measure peripheral blood level of oestradiol and tri-color flow cytometry was applied to counting CD4~+CD25~+Treg cells in 30 patients with RSA (RSA group),30 women of non-pregnancy (non-pregnancy group),and 90 normal pregnant women (early pregnancy,mid-pregnancy and late pregnancy,30 women for each group).Peripheral blood level of oestradiol was detected by electrochemistry immunoluminescence technology for all subjects.Results Compared with that in non-pregnancy group,peripheral blood level of oestradiol was significantly higher in goups of early pregnancy,mid-pregnancy and late pregnancy (t value was respectively 7.42,13.66 and 14.69;all P <0.05);moreover,the longer gestational week was,the higher was the level of oestradiol.However,the oestradiol level of RSA group was markedly lower than that of non-pregnancy group(t=4.11,P<0.05).The number of CD4~+CD25~+Treg of normal pregnancy women(early pregnancy,midpregnancy and late pregnancy)was higher than that of non-gestation women obviously.The number of CD4~+CD25~+Treg was at peak in mid-pregnancy.The number of CD4~+CD25~+Treg was obviously lower in RSA group than that in non-pregnancy group(t=8.07,P<0.05).Conclusion Reduced peripheral blood level of oestradiol and CD4~+CD25~+Treg may down-regulate the immune function of maternal-fetal tolerance,and mediate recurrent spontaneous abortion finally.