畜牧兽医学报
畜牧獸醫學報
축목수의학보
2009年
12期
1823-1825
,共3页
乳腺上皮细胞%成纤维细胞%分离%EGFP基因%猪
乳腺上皮細胞%成纖維細胞%分離%EGFP基因%豬
유선상피세포%성섬유세포%분리%EGFP기인%저
mammary gland epithelial cells%fibroblast ceils%isolation%EGFP gene%porcine
本研究旨在从猪乳腺组织中分离得到上皮细胞和成纤维细胞,并将EGFP基因导入这些细胞.利用乳腺细胞堵养体系从成年猪乳腺组织中分离培养上皮细胞和成纤维细胞,并利用脂质体介导转染技术将EGFP基因导入这些细胞.结果,从成年猪乳腺组织中成功分离培养出上皮细胞和成纤维细胞,获得转EGFP基因上皮细胞和成纤维细胞.上皮细胞呈短梭形或多角形,细胞之间紧密相靠,互相衔接,连接成片;细胞核呈圆形或椭圆形,核仁2～4枚,比较明显.成纤维细胞呈长梭形.结果表明,可以从猪乳腺组织中分离上皮细胞和成纤维细胞,EGFP可以在这些细胞中表达.
本研究旨在從豬乳腺組織中分離得到上皮細胞和成纖維細胞,併將EGFP基因導入這些細胞.利用乳腺細胞堵養體繫從成年豬乳腺組織中分離培養上皮細胞和成纖維細胞,併利用脂質體介導轉染技術將EGFP基因導入這些細胞.結果,從成年豬乳腺組織中成功分離培養齣上皮細胞和成纖維細胞,穫得轉EGFP基因上皮細胞和成纖維細胞.上皮細胞呈短梭形或多角形,細胞之間緊密相靠,互相銜接,連接成片;細胞覈呈圓形或橢圓形,覈仁2～4枚,比較明顯.成纖維細胞呈長梭形.結果錶明,可以從豬乳腺組織中分離上皮細胞和成纖維細胞,EGFP可以在這些細胞中錶達.
본연구지재종저유선조직중분리득도상피세포화성섬유세포,병장EGFP기인도입저사세포.이용유선세포도양체계종성년저유선조직중분리배양상피세포화성섬유세포,병이용지질체개도전염기술장EGFP기인도입저사세포.결과,종성년저유선조직중성공분리배양출상피세포화성섬유세포,획득전EGFP기인상피세포화성섬유세포.상피세포정단사형혹다각형,세포지간긴밀상고,호상함접,련접성편;세포핵정원형혹타원형,핵인2～4매,비교명현.성섬유세포정장사형.결과표명,가이종저유선조직중분리상피세포화성섬유세포,EGFP가이재저사세포중표체.
This experiment was conducted to isolate porcine mammary gland epithelial and fibroblast cells from adult porcine mammary gland tissue, and transfect the enhanced green fluorescent protein (EGFP) gene into the cells. Porcine mammary gland epithelial and fibroblast cells were isolated from adult porcine mammary gland tissue using their culture system. EGFP gene was transfected into the cells by lipofection technology. We succeeded in isolating porcine mammary gland epithelial and fibroblast cells from adult porcine mammary gland tissue, and the EGFP gene transfected epithelial and fibroblast cells were obtained. The epithelial cells were short shuttie-like or guboidal, which were connected with each other closely and properly. The cell nucleus were circular or oval which had 2-4 cores. The fibroblast cells were long shuttle-like. This result shows that porcine epithelial and fibroblast cells could be isolated from adult porcine mammary gland tissue and EGFP could be expressed in the cells.
