CAJ | 학술논문

目的:探讨B细胞趋化因子(B-lymphocyte chemoattractant,BLC)对柯萨奇病毒B3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响.方法:将BALB/c小鼠随机分为4组,分别肌肉注射pcDNA3、pcDNA3/BLC、pcDNA3/C3d3-sVP1和含pcDNA3/BLC与pcDNA3/C3d3-sVP1的混合质粒,于免疫后不同时间检测小鼠血清的中和抗体滴度、特异性CTL杀伤活性;以LD50的CVB3病毒液感染已免疫小鼠,检测小鼠血中病毒滴度,观察存活情况以评价各种疫苗的免疫保护作用.结果:小鼠的中和抗体滴度随免疫次数增加而提高,混合组中和抗体滴度(42.17±1.43)和特异性CTL杀伤率(41.3%±3.51%)均明显高于pcDNA3/C3d3-sVP1组(P＜0.05),而血中病毒滴度低于pcDNA3/C3d3-sVP1组;经致死量CVB3感染后,pcDNA3/BLC和pcDNA3/C3d3-sVP1混合免疫的小鼠生存率达44%,生存率高于其他各组.结论:BLC可显著提高C3d3-sVP1基因诱导的特异性免疫应答,增强基因疫苗对机体的免疫保护作用.
목적:탐토B세포추화인자(B-lymphocyte chemoattractant,BLC)대가살기병독B3융합기인역묘pcDNA3/C3d3-sVP1면역효과적영향.방법:장BALB/c소서수궤분위4조,분별기육주사pcDNA3、pcDNA3/BLC、pcDNA3/C3d3-sVP1화함pcDNA3/BLC여pcDNA3/C3d3-sVP1적혼합질립,우면역후불동시간검측소서혈청적중화항체적도、특이성CTL살상활성;이LD50적CVB3병독액감염이면역소서,검측소서혈중병독적도,관찰존활정황이평개각충역묘적면역보호작용.결과:소서적중화항체적도수면역차수증가이제고,혼합조중화항체적도(42.17±1.43)화특이성CTL살상솔(41.3%±3.51%)균명현고우pcDNA3/C3d3-sVP1조(P＜0.05),이혈중병독적도저우pcDNA3/C3d3-sVP1조;경치사량CVB3감염후,pcDNA3/BLC화pcDNA3/C3d3-sVP1혼합면역적소서생존솔체44%,생존솔고우기타각조.결론:BLC가현저제고C3d3-sVP1기인유도적특이성면역응답,증강기인역묘대궤체적면역보호작용.
Objective:To investigate the influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1.Methods:BALB/c mice were divided into 4 groups randomly, and injected intramuscularly with pcDNA3,pcDNA3/BLC,pcDNA3/C3d3-sVP1 and the combination with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1.At a certain time,they were measured for the titers for neutralizing antibodies,specific CTL cytotoxic activity.The protective efficacy of DNA vaccinations was evaluated by titers of blood viruses and survival rate.Results:The titers for antibodies increased with the time of inoculation.More specifically,the antibody titers (42.17±1.43) and the specific CTL cytotoxic activity (41.3%±3.51%) of the mice in the combination group were remarkably stronger than in the mice with pcDNA3/C3d3-sVP1(P＜0.05),but the virus titers of blood was lower.After lethal CVB3 challenge,the protection of mice from death in the combination group with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1 was 44%.Survival curves indicated that the survive state of combination group was better than others.Conclusion:BLC can strongly enhance the specific immunity induced by C3d3-sVP1.