CAJ | 학술논문

目的探讨透射免疫比浊法与乳胶增强散射免疫比浊法测定ASO、C4定量结果的可比性.方法以日立7170全自动生化分析仪5点定标透射免疫比浊法为参比方法(X),德灵BU prospec特定蛋白分析仪的乳胶增强散射免疫比浊法为待评方法(Y),分别测定高、中值ASO血清各12例.高、中值C4血清各13例.结果经分别平行测定不同水平ASO、C4的混合血清各20次,乳胶增强散射比浊法和透射免疫比浊法的检测精密度均符合实验室要求(＜5%),但前者的重复性(批内变异CV值均小于2%)较后者(CV均小于3%)好;两法分别测定高、中值ASO和高、中值C4定量结果,差异均有统计学意义(P＜0.01),对高、中值ASO和中值C4的相关系数(r)分别为0.982,0.958、0.945,说明两法的相关性良好.而在高值C4两法的相关系数(r)则为0.888.结论乳胶增强散射比浊测定法检测重复性好,灵敏度高,检测范围宽,是一种值得推广应用的较好生化检测方法.
목적 탐토투사면역비탁법여유효증강산사면역비탁법측정ASO、C4정량결과적가비성.방법 이일립7170전자동생화분석의5점정표투사면역비탁법위삼비방법(X),덕령BU prospec특정단백분석의적유효증강산사면역비탁법위대평방법(Y),분별측정고、중치ASO혈청각12례.고、중치C4혈청각13례.결과 경분별평행측정불동수평ASO、C4적혼합혈청각20차,유효증강산사비탁법화투사면역비탁법적검측정밀도균부합실험실요구(＜5%),단전자적중복성(비내변이CV치균소우2%)교후자(CV균소우3%)호;량법분별측정고、중치ASO화고、중치C4정량결과,차이균유통계학의의(P＜0.01),대고、중치ASO화중치C4적상관계수(r)분별위0.982,0.958、0.945,설명량법적상관성량호.이재고치C4량법적상관계수(r)칙위0.888.결론 유효증강산사비탁측정법검측중복성호,령민도고,검측범위관,시일충치득추엄응용적교호생화검측방법.
Objective To compare ASO and CA results from differences between the transmission of immune Turbidimetry latex and enhance immune Turbidimetry scattering.Methods Taking HITACHI 7170 automatic biochemical analyzer with five points calibration as the standard method(X)and Germany Ling BN protein analytical system as the detecting method(Y),1 2 sampies of high,medium level serum ASO and 13 samples of high,medium level serum C4 were measured.The collected data were processed by statistics analysis.Resalts The precision of the transmission of immune Turbidimetry latex and enhance immune Turbidimetry scattering are in line with the requirements of laboratories by testing parallel to the different levels of ASO,C4 mixed the serum 20.But the former is better.The result were significantly different checked by two methods in the high and medium levels of the ASO and C4(P＜0.01),the correlation factors were 0.982、0.958、0.888 and 0.945 respectively.Conclusion Scattering turbidimetry method showed great reproducibility,higher sensitivity,wider detect range,worthing application and generalization.