CAJ | 학술논문

目的研究在冲突性应激条件下大鼠行为和内分泌的变化.方法 56只成年雄性SD大鼠,随机分为8组,每组7只,利用Vogel冲突实验方法稍加修改,作为应激源,开场试验检测行为反应,测量体重,利用酶联免疫方法检测血清中皮质酮的含量.结果与对照组相比,1周、2周、4周应激组大鼠表现为体重明显降低(216.5±8.71g,194.94±14.82g,259.75±13.83g):;在运动性方面,1、2、4周应激组大鼠(67.1±9.2,53.66±6.86,59.25±6.46)有增加的趋势或者明显增加,在实验过程中,所有应激组大鼠皮质酮不同(1次应激组274.41±25.39ng.ml-1,1周应激组240.44±29.51ng.ml-1,2周应激组254.21±37.16ng.ml-1,4周应激组161.02±46ng.ml-1)较对照组(89.33±14.04ng.ml-1)明显升高.结论在应激大鼠表现的增多的运动,升高的皮质酮以及降低的体重,与焦虑障碍的某些特点相似,并且行为和内分泌的变化可以维持一段时间.冲突相关的应激可以作为研究焦虑及其他精神创伤应激比较理想的动物模型.
목적연구재충돌성응격조건하대서행위화내분비적변화.방법 56지성년웅성SD대서,수궤분위8조,매조7지,이용Vogel충돌실험방법초가수개,작위응격원,개장시험검측행위반응,측량체중,이용매련면역방법검측혈청중피질동적함량.결과여대조조상비,1주、2주、4주응격조대서표현위체중명현강저(216.5±8.71g,194.94±14.82g,259.75±13.83g):;재운동성방면,1、2、4주응격조대서(67.1±9.2,53.66±6.86,59.25±6.46)유증가적추세혹자명현증가,재실험과정중,소유응격조대서피질동불동(1차응격조274.41±25.39ng.ml-1,1주응격조240.44±29.51ng.ml-1,2주응격조254.21±37.16ng.ml-1,4주응격조161.02±46ng.ml-1)교대조조(89.33±14.04ng.ml-1)명현승고.결론재응격대서표현적증다적운동,승고적피질동이급강저적체중,여초필장애적모사특점상사,병차행위화내분비적변화가이유지일단시간.충돌상관적응격가이작위연구초필급기타정신창상응격비교이상적동물모형.
Objective To investigate behavioral and endocrinal changes following the conflict-related stress. Method Male SpragueDawley rats were exposed to stressors using Vogel conflict test. Body weight and behavioral responses in open field test were investigated during the whole procedure. Serum corticosterone level was analyzed using immunoassay. Results Compared with the control, the rats of the 1-week, 2-week and 4-week stressed group showed significantly decreased body weight. There was increasing tendency or significant difference in locomotion between the 1 -week, 2-week and 4-week stressed group and their control. And the hormone levels for the stressed animal were reliably high. Conclusions The increased locomotion, and high corticosterone level and reduced body weight in stress animal,was consistent with the features of anxiety-like disorders. Furthermore, the behavioral and endocrine changes produced by conflict-related stress could maintain a long time. The conflictrelated stress paradigm may be used as psychological stress animal mode in the future.