CAJ | 학술논문

目的建立大鼠大脑中动脉阻断(MCAO)模型,颈内动脉局部给予NO前体L-精氨酸(L-Arginine,ARG)、NO供体硝酸甘油(NG),并通过DWI及PWI动态评估大鼠受累脑区血循环与脑损害变化,探讨脑缺血早期ARG和NG的保护作用.方法雄性SD大鼠采用线栓法阻塞右侧大脑中动脉,制作成缺血2 h再灌注模型,并于再灌注即刻患侧颈内动脉分别给予L-ARG、NG,在缺血2 h、再灌3和24 h分别进行T1WI、T2WI、DWI和PWI影像学评估,并与Longa评分、红四氮唑(TTC)染色观察对比.结果给药组DWI高信号区及TTC苍白区较MCAO组减小(P＜0.01);从缺血2 h到再灌24 h,各组大鼠DWI高信号区的ADC、rADC值均呈现升高趋势,再灌3和24 h时,给药组大鼠DWI高信号区的ADC、rADC值比MCAO组升高(P＜0.01).结论 DWI及PWI证实脑缺血超早期NO前体/供体局部介入给药具有显著的脑保护作用.
목적 건립대서대뇌중동맥조단(MCAO)모형,경내동맥국부급여NO전체L-정안산(L-Arginine,ARG)、NO공체초산감유(NG),병통과DWI급PWI동태평고대서수루뇌구혈순배여뇌손해변화,탐토뇌결혈조기ARG화NG적보호작용.방법 웅성SD대서채용선전법조새우측대뇌중동맥,제작성결혈2 h재관주모형,병우재관주즉각환측경내동맥분별급여L-ARG、NG,재결혈2 h、재관3화24 h분별진행T1WI、T2WI、DWI화PWI영상학평고,병여Longa평분、홍사담서(TTC)염색관찰대비.결과 급약조DWI고신호구급TTC창백구교MCAO조감소(P＜0.01);종결혈2 h도재관24 h,각조대서DWI고신호구적ADC、rADC치균정현승고추세,재관3화24 h시,급약조대서DWI고신호구적ADC、rADC치비MCAO조승고(P＜0.01).결론 DWI급PWI증실뇌결혈초조기NO전체/공체국부개입급약구유현저적뇌보호작용.
Objective To establish a rat model of middle cerebral artery occlusion (MCAO)by blockage or obstruction of middle cerebral artery. NO precursor L-Arginine (L-ARG) and NO donator Nitroglycerine (NG)are administrated from intraearotid arteries. DWI and PWI are applied to evaluate blood circulation and brain damage of the effected region to elucidate the piotective function of L-ARG and NG in the early stage of brain ischemia. Methods The middle cerebral artery was occluded by insertion of a suture through the internal carotid artery of SD male rats to duplicate ischemia-reperfusion model. Reperfusion was established by suture withdrawal. After 2 hours of blockage, reperfusion and administrate L-ARG,NG by interventional therapy through the internal carotid artery simultaneously. Image indexes such as T1WI, T2WI, DWI and PWI are utilized to assess the changes in different time points. These indexes, Longa score and TTC stain were compared. Results There were obvious decrease in DWI high signal region and Trc pale region in drugs groups, compared with MCAO group(P＜0.01).ADC and rADC values in DWI high signal region increased gradually from 2 hours after ischemia to 24 hours after reperfusion in each group. ADC and rADC values in DWI high signal region of the drugs groups increased obviously(P＜0.01).Conclusion Interventional therapy with NO precursor/donator showed significant protective function in the early stage of brain ischemia.