国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
23期
2956-2960
,共5页
易峰%孙爱农%廖艳婷%葛峰
易峰%孫愛農%廖豔婷%葛峰
역봉%손애농%료염정%갈봉
献血者%梅毒螺旋体%酶联免疫吸附试验%梅毒螺旋体明胶凝集试验
獻血者%梅毒螺鏇體%酶聯免疫吸附試驗%梅毒螺鏇體明膠凝集試驗
헌혈자%매독라선체%매련면역흡부시험%매독라선체명효응집시험
Blood donor%Treponema pallidum%Enzyme linked immunosorbent assay%Treponema pallidum particle agglutination test
目的 了解2006年1月-2011年8月期间中山地区无偿献血者梅毒感染状况,评价梅毒筛查试剂盒质量,为保证血液安全提供有效的预防措施.方法 用ELISA试剂榆测献血者梅毒螺旋体(TP)抗体,阳性者再用TP明胶凝集试验(TPPA)确认,分析梅毒感染率者的流行病学特征.结果 共检测232 484名无偿献血者TP抗体,阳性1092例(占0.47％)；TP阳性率,在年度间、男女性别、不同采血点、个体与集体无偿献血者间的差别无意义,P值均＞0.05,但不同年龄组差异有极显著性,P＜0.005;初、复检ELISA检测灵敏度已达到99.42％和99.65％,但特异性只有27.81％和63.91％,产生一定的假阳性结果.结论 中山地区无偿献血人群梅毒感染率保持相对稳定水平,无明显上升趋势；对献血者ELISA中TP阳性标本用TPPA确认非常有意义；尽量输注在4℃保存3天以上的血液.
目的 瞭解2006年1月-2011年8月期間中山地區無償獻血者梅毒感染狀況,評價梅毒篩查試劑盒質量,為保證血液安全提供有效的預防措施.方法 用ELISA試劑榆測獻血者梅毒螺鏇體(TP)抗體,暘性者再用TP明膠凝集試驗(TPPA)確認,分析梅毒感染率者的流行病學特徵.結果 共檢測232 484名無償獻血者TP抗體,暘性1092例(佔0.47％)；TP暘性率,在年度間、男女性彆、不同採血點、箇體與集體無償獻血者間的差彆無意義,P值均＞0.05,但不同年齡組差異有極顯著性,P＜0.005;初、複檢ELISA檢測靈敏度已達到99.42％和99.65％,但特異性隻有27.81％和63.91％,產生一定的假暘性結果.結論 中山地區無償獻血人群梅毒感染率保持相對穩定水平,無明顯上升趨勢；對獻血者ELISA中TP暘性標本用TPPA確認非常有意義；儘量輸註在4℃保存3天以上的血液.
목적 료해2006년1월-2011년8월기간중산지구무상헌혈자매독감염상황,평개매독사사시제합질량,위보증혈액안전제공유효적예방조시.방법 용ELISA시제유측헌혈자매독라선체(TP)항체,양성자재용TP명효응집시험(TPPA)학인,분석매독감염솔자적류행병학특정.결과 공검측232 484명무상헌혈자TP항체,양성1092례(점0.47％)；TP양성솔,재년도간、남녀성별、불동채혈점、개체여집체무상헌혈자간적차별무의의,P치균＞0.05,단불동년령조차이유겁현저성,P＜0.005;초、복검ELISA검측령민도이체도99.42％화99.65％,단특이성지유27.81％화63.91％,산생일정적가양성결과.결론 중산지구무상헌혈인군매독감염솔보지상대은정수평,무명현상승추세；대헌혈자ELISA중TP양성표본용TPPA학인비상유의의；진량수주재4℃보존3천이상적혈액.
Objective To explore the current situation of syphilis infection in voluntary blood donors in Zhongshan,to evaluate the quality of syphilis screening kit and to provide effective preventive measures for blood safety.Methods Treponemal antibody was detected by ELISA in all the Blood donors; and those with antibody positive and then comfirmed by TP particle agglutination test ( TPPA ).The epidemiological characteristics of syphilis infection were analyzed.Results During the period of January 2006 to June 2011,a total of 232,484 blood donors were detected for treponemal antibody,1092of whom ( 0.47％ ) were positive.The positive rate of TP did not differ significantly between years,male and female,different blood collection spots,individual and group donors ( P＞ 0.05 ),but differed significantly among different age groups ( P＜ 0.005 ).The sensitivity of initial ELISA was 99.42％ and that of repeat ELISA was 99.65％,but the specificity was only 27.81％ and 63.91％,respectively.Some false positive results were found.Conclusions The rate of syphilis infection in voluntary blood donors in Zhongshan area during January 2006.1 to June 2011.6 remains at a relatively stable level.It is of significance to have TPPA confirm the positive samples detected by ELISA in blood donors.
