CAJ | 학술논문

目的检测调节性T淋巴细胞新旧标志在活动性类风湿关节炎(RA)患者外周血中的表达,探讨用CD127代替FOXP3对调节性T淋巴细胞进行研究的可能性.方法选取活动性RA患者32例,其中未经缓解病情抗风湿药(DMARDs)治疗者20例,经DMARDs治疗效果不佳者12例,以25例原发性干燥综合征(pSS)患者和24名健康人作为对照,采用流式细胞术检测外周血中CD4+CD25high、CD4+CD25+CD127low/-及CD4*CD25+FOXP3+T淋巴细胞的比例,同时检测外周血C反应蛋白(CRP)、红细胞沉降率(ESR)、免疫球蛋白、补体等水平并进行相关性分析.结果 RA患者CD4+CD25highT淋巴细胞百分率与健康对照组相比差异无统计学意义(P>0.05).其CD4+CD25+FOXP3+T淋巴细胞与CD+CD25+CD127low/-T淋巴细胞的百分率均低于健康对照组(P<0.05),但CD4+D25+FOXP3+、CD4+CD25+CD127low/-T淋巴细胞在RA患者与pSS患者中的表达差异无统计学意义(P>0.05);这三群细胞的表达水平在RA未经治疗组和治疗效果不佳组之间差异均无统计学意义(P>0.05). RA患者CD4+CD25+FOXP3+T淋巴细胞与CD4+CD25+CD127low/-T淋巴细胞之间呈明显正相关(r=0.698,P=0.001),但这两群细胞与患者的CRP、ESR等水平及抗环瓜氨酸肽(CCP)抗体、类风湿因子(RF)均无相关性(P>0.05).结论活动性RA患者外周血中,CD4+CD25+CD127low/-T淋巴细胞的百分率降低,并且与CD4+CD25+FOXP3+T淋巴细胞呈显著正相关性,提示CD127可能被作为Treg细胞中FOXP3的替代标记.
목적 검측조절성T림파세포신구표지재활동성류풍습관절염(RA)환자외주혈중적표체,탐토용CD127대체FOXP3대조절성T림파세포진행연구적가능성.방법 선취활동성RA환자32례,기중미경완해병정항풍습약(DMARDs)치료자20례,경DMARDs치료효과불가자12례,이25례원발성간조종합정(pSS)환자화24명건강인작위대조,채용류식세포술검측외주혈중CD4+CD25high、CD4+CD25+CD127low/-급CD4*CD25+FOXP3+T림파세포적비례,동시검측외주혈C반응단백(CRP)、홍세포침강솔(ESR)、면역구단백、보체등수평병진행상관성분석.결과 RA환자CD4+CD25highT림파세포백분솔여건강대조조상비차이무통계학의의(P>0.05).기CD4+CD25+FOXP3+T림파세포여CD+CD25+CD127low/-T림파세포적백분솔균저우건강대조조(P<0.05),단CD4+D25+FOXP3+、CD4+CD25+CD127low/-T림파세포재RA환자여pSS환자중적표체차이무통계학의의(P>0.05);저삼군세포적표체수평재RA미경치료조화치료효과불가조지간차이균무통계학의의(P>0.05). RA환자CD4+CD25+FOXP3+T림파세포여CD4+CD25+CD127low/-T림파세포지간정명현정상관(r=0.698,P=0.001),단저량군세포여환자적CRP、ESR등수평급항배과안산태(CCP)항체、류풍습인자(RF)균무상관성(P>0.05).결론 활동성RA환자외주혈중,CD4+CD25+CD127low/-T림파세포적백분솔강저,병차여CD4+CD25+FOXP3+T림파세포정현저정상관성,제시CD127가능피작위Treg세포중FOXP3적체대표기.
Objective To investigate the expression of regulatory T cells in the peripheral blood of rheumatoid arthritis patients and to explore the possibility of using CD127 instead of FOXP3 in the study of regulatory T cells. Methods Thirty-two patients with active rheumatoid arthritis (RA) [ including 20 patients who hadn't been treated with disease modifying antirheumatic drugs (DMARDs) and 12 patients whose disease were poorly improved after DMARDs therapy], 25 patients with primary Sjogren's syndrome (pSS) and 24 healthy controls were selected. The percentages of CD4+CD25high, CD4+CD25+CD127low/'- and CD4+CD25+ FOXP3+T cells were analyzed by 3-color flow cytometry. Meanwhile, the levels of CRP, ESR, immunoglobulin and complement were measured. Results Percentages of CD4+CD25+CD127low/-T cells and CD4+CD25+FOXP3+ T cells in RA patients were significantly lower than those of healthy controls (P<0.01 for each category of T cells),but the percentage of CD4+CD25high T cells didn't show significant changes (P>0.05). The expression of CD4+CD25+CD127ow/- and CD4+CD25+FOXP3+T cells showed no difference between RA and pSS patients (P> 0.05 for each). Differences of the three group cells between untreated RA patients and poorly-improved RA patients were not significant (P>0.05 for each case). CD4+CD25+CD127low/-T cells were positively associated with CD4+CD25+FOXP3+T cells (r=0.698, P=0.001), but the two groups had no correlation with the levels of CRP, ESR and anti-CCP antibody and RF (P>0.05 for each case). Conclusion The percentage of CD4+CD25+CD127low/-T cells is decreased in the peripheral blood of active RA patients and positively correlates with CD4+CD25+FOXP3+T cells.It suggests that CD127 may be an ahemative marker of FOXP3 in the study of Treg cells.