中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
2期
78-80
,共3页
吴易%蒙金秋%严煜林%曹存巍%刘栋华%梁伶
吳易%矇金鞦%嚴煜林%曹存巍%劉棟華%樑伶
오역%몽금추%엄욱림%조존외%류동화%량령
荨麻疹%等位基因%基因,MHCⅡ类%皮肤试验
蕁痳疹%等位基因%基因,MHCⅡ類%皮膚試驗
담마진%등위기인%기인,MHCⅡ류%피부시험
Urticaria%Alleles%Genes,MHC class Ⅱ%Skin tests
目的 探讨广西地区白体血清皮肤试验阳性慢性荨麻疹与HLA-DRB1等位基因遗传易感性的关系.方法 对144例广西地区慢性荨麻疹患者进行自体血清皮肤试验,按试验结果分为阳性组62例,阴性组82例.采用聚合酶链反应-序列特异性引物方法,对患者组和199例正常人对照组进行HLA-DRB1等位基因的分型,并分析DRB1基因在3个组中的分布.使用SPSS13.0统计软件分析.结果 DRB1*01、*1401、*16等位基因频率在阳性组、阴性组和正常人对照组间比较,差异均有统计学意义(x2=10.92,Pc=0.03;x2=35.34,Pc<0.01;x2=12.69,Pc=0.03).进一步在各组间进行两两比较,仅DRB1*1401等位基因频率在自体血清皮肤试验阳性组与对照组间(RR=17.09,Pc<0.01)及自体血清皮肤试验阳性组与阴性组间(RR=7.20,P<0.01),差异均有统计学意义.结论 DRB1*1401等位基因可能是广西地区自体血清皮肤试验阳性慢性荨麻疹的易感基因或与其连锁.
目的 探討廣西地區白體血清皮膚試驗暘性慢性蕁痳疹與HLA-DRB1等位基因遺傳易感性的關繫.方法 對144例廣西地區慢性蕁痳疹患者進行自體血清皮膚試驗,按試驗結果分為暘性組62例,陰性組82例.採用聚閤酶鏈反應-序列特異性引物方法,對患者組和199例正常人對照組進行HLA-DRB1等位基因的分型,併分析DRB1基因在3箇組中的分佈.使用SPSS13.0統計軟件分析.結果 DRB1*01、*1401、*16等位基因頻率在暘性組、陰性組和正常人對照組間比較,差異均有統計學意義(x2=10.92,Pc=0.03;x2=35.34,Pc<0.01;x2=12.69,Pc=0.03).進一步在各組間進行兩兩比較,僅DRB1*1401等位基因頻率在自體血清皮膚試驗暘性組與對照組間(RR=17.09,Pc<0.01)及自體血清皮膚試驗暘性組與陰性組間(RR=7.20,P<0.01),差異均有統計學意義.結論 DRB1*1401等位基因可能是廣西地區自體血清皮膚試驗暘性慢性蕁痳疹的易感基因或與其連鎖.
목적 탐토엄서지구백체혈청피부시험양성만성담마진여HLA-DRB1등위기인유전역감성적관계.방법 대144례엄서지구만성담마진환자진행자체혈청피부시험,안시험결과분위양성조62례,음성조82례.채용취합매련반응-서렬특이성인물방법,대환자조화199례정상인대조조진행HLA-DRB1등위기인적분형,병분석DRB1기인재3개조중적분포.사용SPSS13.0통계연건분석.결과 DRB1*01、*1401、*16등위기인빈솔재양성조、음성조화정상인대조조간비교,차이균유통계학의의(x2=10.92,Pc=0.03;x2=35.34,Pc<0.01;x2=12.69,Pc=0.03).진일보재각조간진행량량비교,부DRB1*1401등위기인빈솔재자체혈청피부시험양성조여대조조간(RR=17.09,Pc<0.01)급자체혈청피부시험양성조여음성조간(RR=7.20,P<0.01),차이균유통계학의의.결론 DRB1*1401등위기인가능시엄서지구자체혈청피부시험양성만성담마진적역감기인혹여기련쇄.
Objective To investigate the relationship between HLA-DRB1 alleles and chronic urticaria with positive autologous serum skin test (ASST) in Guangxi Zhuang Autonomous Region.Methods ASST was conducted in 144 patients with chronic urticaria,who were subsequently divided into two groups according to the test result:positive group (n =62) and negative group (n =82).PCR amplification with sequence-specific primers was used to determine the genotypes of HLA-DRB1 alleles in the patients and 199 normal human controls.Chi-square test was performed to analyse the difference in the frequency of HLA-DRB1 alleles between the 3 groups by using the SPSS 13.0 statistical software package.Results There were significant differences in the frequency of HLA-DRB1*01,*1401 and *16 alleles among the patients with positive and negative ASST and the controls (x2 =10.92,Pc =0.032;x2 =35.34,Pc < 0.01 ;x2 =12.69,Pc =0.032).Paired comparison revealed significant differences in the frequency of HLA-DRB1*1401 allele between the patients with positive ASST and controls (RR =17.09,Pc < 0.01 ) as well as between the patients with positive and negative ASST (RR =7.20,Pc < 0.01).Conclusion HLA-DRB1*1401 allele may be,or be linked to,the predisposing gene of chronic urticaria with positive ASST in Guangxi Zhuang Autonomous Region.