中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2009年
1期
31-34
,共4页
甄勇%罗祺%许侃%陈学思%赵长稳%王柏%张德智%王宏磊%徐宁%于金路
甄勇%囉祺%許侃%陳學思%趙長穩%王柏%張德智%王宏磊%徐寧%于金路
견용%라기%허간%진학사%조장은%왕백%장덕지%왕굉뢰%서저%우금로
壳聚糖%甘油磷酸钠%水凝胶%动脉瘤%栓塞材料
殼聚糖%甘油燐痠鈉%水凝膠%動脈瘤%栓塞材料
각취당%감유린산납%수응효%동맥류%전새재료
Chitosan%Glycerophosphate%Hydrogel%Aneurysms%Embolism materials
目的 探讨壳聚糖/甘油磷酸钠水凝胶作为动脉瘤栓塞材料的可行性.方法 配制2%壳聚糖与56%甘油磷酸钠溶液,不同比例混合,测定混合液pH、37℃成胶时间及力学强度,选择最佳比例.体外栓塞动脉瘤模型,评价导管内操控性;通过兔耳中动脉栓塞评价组织相容性.最终栓塞兔动脉瘤模型.结果 当壳聚糖/甘油磷酸钠在7∶1时,pH值为7.28,成胶时间为(260±18)s,力学强度可达14 kPa.体外动脉瘤模型栓塞见壳聚糖/甘油磷酸钠水凝胶可通过导管在动脉瘤内成胶.兔耳中动脉栓塞后3 d,血管周围可见少量炎性细胞浸润,1周时达到高峰,2-3周逐渐消退,4-8周形成肉芽组织,并可见壳聚糖凝胶部分降解.兔动脉瘤栓塞术中见动脉瘤不显影,术后3 d病理见动脉瘤被凝胶栓塞,内膜细胞形态正常,动脉壁内未见明显炎性细胞浸润.结论 短期观察,壳聚糖/甘油磷酸钠水凝胶可用于动脉瘤栓塞.
目的 探討殼聚糖/甘油燐痠鈉水凝膠作為動脈瘤栓塞材料的可行性.方法 配製2%殼聚糖與56%甘油燐痠鈉溶液,不同比例混閤,測定混閤液pH、37℃成膠時間及力學彊度,選擇最佳比例.體外栓塞動脈瘤模型,評價導管內操控性;通過兔耳中動脈栓塞評價組織相容性.最終栓塞兔動脈瘤模型.結果 噹殼聚糖/甘油燐痠鈉在7∶1時,pH值為7.28,成膠時間為(260±18)s,力學彊度可達14 kPa.體外動脈瘤模型栓塞見殼聚糖/甘油燐痠鈉水凝膠可通過導管在動脈瘤內成膠.兔耳中動脈栓塞後3 d,血管週圍可見少量炎性細胞浸潤,1週時達到高峰,2-3週逐漸消退,4-8週形成肉芽組織,併可見殼聚糖凝膠部分降解.兔動脈瘤栓塞術中見動脈瘤不顯影,術後3 d病理見動脈瘤被凝膠栓塞,內膜細胞形態正常,動脈壁內未見明顯炎性細胞浸潤.結論 短期觀察,殼聚糖/甘油燐痠鈉水凝膠可用于動脈瘤栓塞.
목적 탐토각취당/감유린산납수응효작위동맥류전새재료적가행성.방법 배제2%각취당여56%감유린산납용액,불동비례혼합,측정혼합액pH、37℃성효시간급역학강도,선택최가비례.체외전새동맥류모형,평개도관내조공성;통과토이중동맥전새평개조직상용성.최종전새토동맥류모형.결과 당각취당/감유린산납재7∶1시,pH치위7.28,성효시간위(260±18)s,역학강도가체14 kPa.체외동맥류모형전새견각취당/감유린산납수응효가통과도관재동맥류내성효.토이중동맥전새후3 d,혈관주위가견소량염성세포침윤,1주시체도고봉,2-3주축점소퇴,4-8주형성육아조직,병가견각취당응효부분강해.토동맥류전새술중견동맥류불현영,술후3 d병리견동맥류피응효전새,내막세포형태정상,동맥벽내미견명현염성세포침윤.결론 단기관찰,각취당/감유린산납수응효가용우동맥류전새.
Objective To evaluate the feasibility of injectable thermosetting chitosan/glycerophosphate(C/GP)hydrogel as the embolism material of aneurysms.Method 2%chitosan solution and 56%glycerophosphate solution were mixed in different volume ratios.The pH,colloidization time in 37℃and mechanic strength of the mixed solution were detected respectively to determine the best ratio.The intracatheter operation of the C/GP hydrogel by embolizing aneurysm models was evaluated.The C/GP hydrogel histocompatibility was evaluated by embolizing rabbit ear arteries. Finally,we embolized rabbit aneurysm models with this C/GP gel.Results When the ratio of C/GP was 7:1 at pH 7.28,the colloidization time was(260±18)seconds and the mechanic strength chould reach 14 kPa.In the experiment of embolizing aneurysm models in vitro,the C/GP gel could arrive in aneurysms through catheter and reach solid state.In the experiment of embolizing rabbit ear arteries,the modest inflammation was observed.This effect peak was found in 1 week,and gradually resolution in 2-3 weeks.In 4-8 weeks,granulation tissue and slight degradation of C/GP hydrogel could be observed.In the embolism operation of rabbit aneurysms,the aneurysms were disappeared in angiography.In the HE stain slice 3 days after operation,the C/GP hydrogel in aneurysm caves and normal endomembrane cells were observed.There was no significant flame cell in aneurysm walls.Conclusions In short term view,C/GP hydrogel could be used to embolize aneurysms.
