CAJ | 학술논문

目的观察人髓样细胞触发性受体(TREM)-1在急性胆管炎患者中表达的规律及其临床意义,探讨TREM-1在急性胆管炎中与肿瘤坏死因子(TNF)-á,CRP的相关性.方法分别收集34例急性胆管炎患者(AC),24例急性重症胆管炎(ACST)患者和60例正常人外周血白细胞,以半定量逆转录.聚合酶链反应(RT-PCR)检测中性粒细胞中THEM-1的mRNA表达;免疫细胞化学检测TREM-1的蛋白表达.结果对照组TREM-1的mRNA半定量比值为0.458±0.043.AC和ACST组治疗前分别为0.809±0.044、0.981±0.089,术后24 h为0.785±0.077、0.954±0.072,48 h为0.565±0.009、0.719±0.047,ACST的TREM-1表达水平明显高于AC(P＜0.05).免疫细胞化学镜下观察结果与RT-PCR结果一致.TREM-1与TNF-á,CRP表达水平高度正相关(r1=0.719,r2=0.701,P＜0.01).结论人TREM-1在急性胆管炎发病初表达增高,与TNF-á,CRP表达高度正相关.提示TREM-1在急性胆管炎的发生发展中起重要作用.
목적 관찰인수양세포촉발성수체(TREM)-1재급성담관염환자중표체적규률급기림상의의,탐토TREM-1재급성담관염중여종류배사인자(TNF)-á,CRP적상관성.방법 분별수집34례급성담관염환자(AC),24례급성중증담관염(ACST)환자화60례정상인외주혈백세포,이반정량역전록.취합매련반응(RT-PCR)검측중성립세포중THEM-1적mRNA표체;면역세포화학검측TREM-1적단백표체.결과 대조조TREM-1적mRNA반정량비치위0.458±0.043.AC화ACST조치료전분별위0.809±0.044、0.981±0.089,술후24 h위0.785±0.077、0.954±0.072,48 h위0.565±0.009、0.719±0.047,ACST적TREM-1표체수평명현고우AC(P＜0.05).면역세포화학경하관찰결과 여RT-PCR결과 일치.TREM-1여TNF-á,CRP표체수평고도정상관(r1=0.719,r2=0.701,P＜0.01).결론 인TREM-1재급성담관염발병초표체증고,여TNF-á,CRP표체고도정상관.제시TREM-1재급성담관염적발생발전중기중요작용.
Objective To investigate the expression of human triggering receptor on myeloid cells-1 (TREM-1) and its regularity or clinical significance in patients with acute cholangitis,and discuss the relationship of TREM-I ,TNF-α and CRP in acute cholangitis. Methods Peripheral blood of 24 patients with ACST,34 patients with AC and 60 health adults were collected respectively. TREM-1 mRNA and protein was detected by semi-quantitative RT-PCR and immunocytochemistry respectively. Results The values of TREM-1/β-actin in control group was 0. 458 ± 0. 043, while those in AC and ACST group were 0.809 ± 0.044 and 0.981 ± 0.089 before therapy, and 0.785 ± 0. 077 ,0. 954 ± 0. 072 at 24 h, 0. 565 ± 0. 009 , 0. 719 ± 0.047 at 48 h, respectively. The level of TREM-1 express in ACST was higher than in AC (P ＜ 0.05). Immunocytochemistry revealed the results similar to those of RT-PCR. TREM-I expression was closely related with TNF-α, CRP expression ( r1 = 0. 719, r2 = 0. 701, P ＜ 0.01 ). Conclusion Expression of human TREM-1 was up-regulated obviously in patients with AC and ACST in the early stage,indicating that TREM-1 might play an important role in the development of acute eholangitis.