CAJ | 학술논문

目的研究18号染色体短臂的杂合性缺失在膀胱癌发生发展中的作用，为早诊及定位克隆相关基因提供资料。方法利用位于18q上的12个多态性微卫星位点进行缺失做图，并对此区域的抑癌基因Smad 2和Sma d 4进行免疫组织化学分析。结果 84.2%（32/38）的病例至少在12个位点中的一个发生杂合性缺失，最小缺失区域跨越Smad 4基因。免疫组化结果显示：Smad 2蛋白有21.1%（8/38）表达上调，34.2%（13/38）表达下调；Smad 4蛋白有68.4%（26/38）表达下调而仅1例表达上调。结论 18号染色体短臂的缺失与膀胱癌变相关。位于18号染色体短臂的两个候选抑癌基因Smad 2和 Smad 4的异常表达，对于膀胱癌的发生、发展可能有着重要作用。
목적연구18호염색체단비적잡합성결실재방광암발생발전중적작용，위조진급정위극륭상관기인제공자료。방법이용위우18q상적12개다태성미위성위점진행결실주도，병대차구역적억암기인Smad 2화Sma d 4진행면역조직화학분석。결과 84.2%（32/38）적병례지소재12개위점중적일개발생잡합성결실，최소결실구역과월Smad 4기인。면역조화결과현시：Smad 2단백유21.1%（8/38）표체상조，34.2%（13/38）표체하조；Smad 4단백유68.4%（26/38）표체하조이부1례표체상조。결론 18호염색체단비적결실여방광암변상관。위우18호염색체단비적량개후선억암기인Smad 2화 Smad 4적이상표체，대우방광암적발생、발전가능유착중요작용。
Objective　To investigate the rol e of loss of heterozygosity (LOH) on chromosome 18q in the carcinogenesis and pr ogression of urinary bladder cancer and to provide clues for early detection and positional cloning of related genes.　Methods　Deletion mapping was performed on 18q using 12 microsatellite markers. Immun ohistochemistry staining was used to evaluate the expression level of two tumor suppressor candidates Smad 2 and Smad 4 in this region.　Res ults　LOH in at least one of the 12 microsatellites on 18q was d etected in 84.2% (32/38) of patients with bladder cancer.The minimal deletion region included tumor suppressor candidate Smad 4. Immunohistochemical study rev ealed that 21.1% (8/38) of the cases had up-regulated Smad 2 protein and 34.2 % (13/38) of the cases had down-regulated Smad 2 protein. Smad4 protein express ion was down-regulated in 68.4% (26/38) of the cases but up-regulated 　S mad 4 expression was seen in only one case.　 Conclusions[ WT5”BZ〗　LOH on 18q was closely linked with 　bladder cancer. The 　irregular 　expression of tumor suppressor 　candidates Smad 2 and Sm ad 4 may play important role in the initiation and progression of bladder neopla sms.