CAJ | 학술논문

目的观察运动训练对脑出血大鼠神经细胞凋亡基因Bcl-2、Bax及其蛋白表达的的影响.方法共选取健康雄性SD大鼠120只,将其随机分为3组.实验组大鼠采用胶原酶诱导法制作大鼠脑出血模型;对照组大鼠手术操作同实验组,但术后不给予运动训练;假手术组大鼠正常饲养.实验组大鼠于术后24 h开始跑笼训练,其余大鼠则在标准笼内自由活动.各组大鼠分别于术后第7,14,21及28天时各取5只用于免疫组化检测,另取5只用于RT-PCR及Western blotting检测.结果 ①免疫组化结果:大鼠Bel-2、Bax阳性细胞胞浆均呈棕黄色,阳性神经元主要分布于血肿周围及大脑皮质区域.实验组大鼠Bcl-2表达水平于术后第21天时开始上升,与假手术组比较,差异有统计学意义(P＜0.01),于术后28 d时达到峰值,此时与对照组及假手术组比较,差异均有统计学意义(P＜0.01).实验组大鼠Bax表达水平于术后7 d时开始下降,术后14～21 d下降显著,术后28 d时虽有回升趋势,但与对照组和假手术组比较,差异仍有统计学意义(P＜0.05).②Western blotting及RT-PCB检测结果:各组大鼠检测结果与免疫组化检测结果基本一致,但实验组大鼠Bel-2 mRNA和Bax mRNA表达高峰时间均较相应蛋白表达高峰时间提前.结论运动训练可上调脑出血大鼠Bcl-2基因及其蛋白表达,下调Bax基因及其蛋白表达,从而抑制脑出血后细胞凋亡,促进功能恢复.
목적 관찰운동훈련대뇌출혈대서신경세포조망기인Bcl-2、Bax급기단백표체적적영향.방법 공선취건강웅성SD대서120지,장기수궤분위3조.실험조대서채용효원매유도법제작대서뇌출혈모형;대조조대서수술조작동실험조,단술후불급여운동훈련;가수술조대서정상사양.실험조대서우술후24 h개시포롱훈련,기여대서칙재표준롱내자유활동.각조대서분별우술후제7,14,21급28천시각취5지용우면역조화검측,령취5지용우RT-PCR급Western blotting검측.결과 ①면역조화결과:대서Bel-2、Bax양성세포포장균정종황색,양성신경원주요분포우혈종주위급대뇌피질구역.실험조대서Bcl-2표체수평우술후제21천시개시상승,여가수술조비교,차이유통계학의의(P＜0.01),우술후28 d시체도봉치,차시여대조조급가수술조비교,차이균유통계학의의(P＜0.01).실험조대서Bax표체수평우술후7 d시개시하강,술후14～21 d하강현저,술후28 d시수유회승추세,단여대조조화가수술조비교,차이잉유통계학의의(P＜0.05).②Western blotting급RT-PCB검측결과:각조대서검측결과여면역조화검측결과기본일치,단실험조대서Bel-2 mRNA화Bax mRNA표체고봉시간균교상응단백표체고봉시간제전.결론 운동훈련가상조뇌출혈대서Bcl-2기인급기단백표체,하조Bax기인급기단백표체,종이억제뇌출혈후세포조망,촉진공능회복.
Objective To investigate the effects of therapeutic exercise(cage running)on the expression of Bel-2 and Bax in neuronal cells after cerebral hemorrhage(ICH)in rats. Methods A total of 120 male Sprague-Dawley rats weighing 270 to 300 g were divided into a trial group(ICH and exercise group,n=40),a control group (ICH only,n=40)and a sham-operated group(sham ICH operation and no exercise,n=40).The brains were removed at 7,14,21 and 28 days after ICH.The activation of Bcl-2 and Bax WaS measured by immunohistochemical techniques,Western blotting and reverse transcriptase polymerase chain reaction(RT-PCR).Results (1)Bel-2-positive and Bax-positive cells appeared in tissues surrounding the hematoma in the cortex.The number of Bcl-2-posotive and Bax-positive cells was nearly zero in the sham-operated group.In the trim group.the number of Bcl-2-positive cells increased significantly from the 21 st to the 28th day.Bax.positive cells decreased from the 7th to the 28th day after ICH when compared with the control group and the sham-operated group.(2)The expression of Bcl-2mRNA and Bax-mRNA as shown by Western blotting and RT-PCR methods was synchronous with the immunohistochemistry results.The peaks of Bcl-2mRNA and BaxmRNA expression were a little earlier than those of Bcl-2 and Bax proteins. Conclusion Exercise can mitigate neuronal cell apoptosis by upregulating the expression of Bcl-2 and Bcl-2 mRNA,and downregulating the expression of Bax and Bax mRNA.