中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2012年
5期
436-442
,共7页
段秀枝%沈默%韩秀翠%吴秀玲%万丽%翁志梁%沈龙捷%陈映鹤%蔡健%余志贤%郑元财%陶志华
段秀枝%瀋默%韓秀翠%吳秀玲%萬麗%翁誌樑%瀋龍捷%陳映鶴%蔡健%餘誌賢%鄭元財%陶誌華
단수지%침묵%한수취%오수령%만려%옹지량%침룡첩%진영학%채건%여지현%정원재%도지화
膀胱肿瘤%膀胱炎%淋巴毒素β受体%转录因子RelA
膀胱腫瘤%膀胱炎%淋巴毒素β受體%轉錄因子RelA
방광종류%방광염%림파독소β수체%전록인자RelA
Urinary bladder neoplasms%Cystitis%Lymphotoxin beta receptor%Transcription factor RelA
目的 探讨淋巴毒素β受体(LTβR)和核转录因子(NF)-κB经典通路因子P65在膀胱癌(BCa)和膀胱炎组织中的表达情况及与BCa临床病理指征的关系.方法 采用实时荧光定量PCR 检测108份新鲜膀胱组织标本(按照病理检查结果分为BCa组75份、膀胱炎组10份和正常膀胱黏膜组23份)中的LTβR和P65的mRNA相对含量；采用免疫组织化学方法检测118份病理石蜡切片(BCa切片组73份、膀胱炎切片组30份和正常膀胱黏膜切片组15份)中的LTβR与磷酸化蛋白P65( p-P65)表达水平,并分析其表达与BCa分级分期和淋巴结转移等因素的关系.采用Krukal-Wallis H检验和x2检验等比较不同膀胱组织间的LTβR与P65的mRNA和蛋白表达水平.结果 (1) LTβR和P65 mRNA相对含量在膀胱癌组中高于正常组[LTβR:29.4 (14.2～46.7)×10-3、1.2 (0.3 ～7.0) ×10-3,Z=-5.508; P65:9.7(2.7 ～21.1)×10-3、1.0(0.8～1.8) ×10-3,Z=-5.030,P均＜0.05],两者在BCa进展[病理分级各组(Ⅰ、Ⅱ、Ⅲ级):LTβR:18.2(2.1 ～31.3) ×10-3、28.4( 16.6 ～36.2)×10-3、47.9 (34.3 ～70.5)×10-3,x2K-W=20.378; P65:4.9(1.3～12.0)×10-3、7.4(3.0～21.9) ×10-3、17.0(10.0～28.3) ×10-3,x2K-W=15.219,P均＜0.05]和淋巴结转移各组(阴性、阳性)[LTβR:27.2(9.7 ～ 40.1)×10-3、39.4(26.7 ～52.6)×10-3,Z=-2.552;P65:7.4(2.3～15.6)×10-3、13.4(6.7～23.3) ×10-3,Z=-2.026,P均＜0.05]比较差异均有统计学意义.(2) LTβR和p-P65蛋白阳性率在膀胱癌组中高于正常组(LTβR:69.8％、13.3％,x2=16.600; P65:56.2％、6.7％,x2=12.220,P均＜0.05).LTβR和p-P65蛋白阳性率也在BCa进展(病理分级各组(Ⅰ、Ⅱ、Ⅲ级):LTβR:56.3％、70.0％、90.4％,x2 =7.055; p-P65:40.6％、60.0％、76.2％,x2=6.679,P均＜0.05)和淋巴结转移各组(阴性、阳性)(LTβR:58.3％、92.0％,x2=8.849; p-P65:52.1％、64.0％,x2=5.088,P均＜0.05)比较差异均有统计学意义.(3)相关性分析显示,LTβR与P65的表达在膀胱癌组中呈正相关(mRNA:r =0.654,P＜0.05；蛋白:r=0.399,P＜0.05),LTβR与65蛋白表达在膀胱炎组中呈正相关(r=0.521,P＜0.05).结论 LTβR和P65在膀胱癌组织中高表达,LTβR的表达可能与膀胱癌的发生发展和转移有关,且LTβR可能通过P65的激活参与经典的NF-κB通路.
目的 探討淋巴毒素β受體(LTβR)和覈轉錄因子(NF)-κB經典通路因子P65在膀胱癌(BCa)和膀胱炎組織中的錶達情況及與BCa臨床病理指徵的關繫.方法 採用實時熒光定量PCR 檢測108份新鮮膀胱組織標本(按照病理檢查結果分為BCa組75份、膀胱炎組10份和正常膀胱黏膜組23份)中的LTβR和P65的mRNA相對含量；採用免疫組織化學方法檢測118份病理石蠟切片(BCa切片組73份、膀胱炎切片組30份和正常膀胱黏膜切片組15份)中的LTβR與燐痠化蛋白P65( p-P65)錶達水平,併分析其錶達與BCa分級分期和淋巴結轉移等因素的關繫.採用Krukal-Wallis H檢驗和x2檢驗等比較不同膀胱組織間的LTβR與P65的mRNA和蛋白錶達水平.結果 (1) LTβR和P65 mRNA相對含量在膀胱癌組中高于正常組[LTβR:29.4 (14.2～46.7)×10-3、1.2 (0.3 ～7.0) ×10-3,Z=-5.508; P65:9.7(2.7 ～21.1)×10-3、1.0(0.8～1.8) ×10-3,Z=-5.030,P均＜0.05],兩者在BCa進展[病理分級各組(Ⅰ、Ⅱ、Ⅲ級):LTβR:18.2(2.1 ～31.3) ×10-3、28.4( 16.6 ～36.2)×10-3、47.9 (34.3 ～70.5)×10-3,x2K-W=20.378; P65:4.9(1.3～12.0)×10-3、7.4(3.0～21.9) ×10-3、17.0(10.0～28.3) ×10-3,x2K-W=15.219,P均＜0.05]和淋巴結轉移各組(陰性、暘性)[LTβR:27.2(9.7 ～ 40.1)×10-3、39.4(26.7 ～52.6)×10-3,Z=-2.552;P65:7.4(2.3～15.6)×10-3、13.4(6.7～23.3) ×10-3,Z=-2.026,P均＜0.05]比較差異均有統計學意義.(2) LTβR和p-P65蛋白暘性率在膀胱癌組中高于正常組(LTβR:69.8％、13.3％,x2=16.600; P65:56.2％、6.7％,x2=12.220,P均＜0.05).LTβR和p-P65蛋白暘性率也在BCa進展(病理分級各組(Ⅰ、Ⅱ、Ⅲ級):LTβR:56.3％、70.0％、90.4％,x2 =7.055; p-P65:40.6％、60.0％、76.2％,x2=6.679,P均＜0.05)和淋巴結轉移各組(陰性、暘性)(LTβR:58.3％、92.0％,x2=8.849; p-P65:52.1％、64.0％,x2=5.088,P均＜0.05)比較差異均有統計學意義.(3)相關性分析顯示,LTβR與P65的錶達在膀胱癌組中呈正相關(mRNA:r =0.654,P＜0.05；蛋白:r=0.399,P＜0.05),LTβR與65蛋白錶達在膀胱炎組中呈正相關(r=0.521,P＜0.05).結論 LTβR和P65在膀胱癌組織中高錶達,LTβR的錶達可能與膀胱癌的髮生髮展和轉移有關,且LTβR可能通過P65的激活參與經典的NF-κB通路.
목적 탐토림파독소β수체(LTβR)화핵전록인자(NF)-κB경전통로인자P65재방광암(BCa)화방광염조직중적표체정황급여BCa림상병리지정적관계.방법 채용실시형광정량PCR 검측108빈신선방광조직표본(안조병리검사결과분위BCa조75빈、방광염조10빈화정상방광점막조23빈)중적LTβR화P65적mRNA상대함량；채용면역조직화학방법검측118빈병리석사절편(BCa절편조73빈、방광염절편조30빈화정상방광점막절편조15빈)중적LTβR여린산화단백P65( p-P65)표체수평,병분석기표체여BCa분급분기화림파결전이등인소적관계.채용Krukal-Wallis H검험화x2검험등비교불동방광조직간적LTβR여P65적mRNA화단백표체수평.결과 (1) LTβR화P65 mRNA상대함량재방광암조중고우정상조[LTβR:29.4 (14.2～46.7)×10-3、1.2 (0.3 ～7.0) ×10-3,Z=-5.508; P65:9.7(2.7 ～21.1)×10-3、1.0(0.8～1.8) ×10-3,Z=-5.030,P균＜0.05],량자재BCa진전[병리분급각조(Ⅰ、Ⅱ、Ⅲ급):LTβR:18.2(2.1 ～31.3) ×10-3、28.4( 16.6 ～36.2)×10-3、47.9 (34.3 ～70.5)×10-3,x2K-W=20.378; P65:4.9(1.3～12.0)×10-3、7.4(3.0～21.9) ×10-3、17.0(10.0～28.3) ×10-3,x2K-W=15.219,P균＜0.05]화림파결전이각조(음성、양성)[LTβR:27.2(9.7 ～ 40.1)×10-3、39.4(26.7 ～52.6)×10-3,Z=-2.552;P65:7.4(2.3～15.6)×10-3、13.4(6.7～23.3) ×10-3,Z=-2.026,P균＜0.05]비교차이균유통계학의의.(2) LTβR화p-P65단백양성솔재방광암조중고우정상조(LTβR:69.8％、13.3％,x2=16.600; P65:56.2％、6.7％,x2=12.220,P균＜0.05).LTβR화p-P65단백양성솔야재BCa진전(병리분급각조(Ⅰ、Ⅱ、Ⅲ급):LTβR:56.3％、70.0％、90.4％,x2 =7.055; p-P65:40.6％、60.0％、76.2％,x2=6.679,P균＜0.05)화림파결전이각조(음성、양성)(LTβR:58.3％、92.0％,x2=8.849; p-P65:52.1％、64.0％,x2=5.088,P균＜0.05)비교차이균유통계학의의.(3)상관성분석현시,LTβR여P65적표체재방광암조중정정상관(mRNA:r =0.654,P＜0.05；단백:r=0.399,P＜0.05),LTβR여65단백표체재방광염조중정정상관(r=0.521,P＜0.05).결론 LTβR화P65재방광암조직중고표체,LTβR적표체가능여방광암적발생발전화전이유관,차LTβR가능통과P65적격활삼여경전적NF-κB통로.
Objective To investigate the relationship of lymphotoxin β receptor (LTβR) and classical nuclear factor-κB (NF-κB) activation pathway in the pathogenesis and progress of cystitis and bladder cancer.Methods The LTβR and P65 mRNA expression were detected by Real-time quantitative PCR in 108 cases of fresh bladder tissue specimens (75 cases of bladder cancer,10 cases of inflammation and 23 normal bladder mucosa cases grouped by the tissue classification ),and protein expression were analyzed by immunohistochemistry assay in 118 cases of paraffin-embedded biopsy specimens (73 cases of bladder cancer,30 cases of cysitis and 15 normal bladder mucosa cases).The correlation analysis between the expressions of LTβR and P65 with clinical pathological data was then performed.Differences between LTβR and P65 mRNA and protein expression level were compared in different groups of bladder tissues using Kruskal-Wallis H test and the Chi-square test.Results ( 1 )The mRNA expressions of LTβR and NF-κB/P65were higher in bladder cancer than those in normal group ( LTβR:29.4 ( 14.2 - 46.7 ) × 10 - 3/1.2 ( 0.3 -7.0) ×10-3,Z=-5.508; P65:9.7 (2.7 -21.1) ×10-3/1.0(0.8 ～1.8) ×10-3,Z=-5.030,P＜0.05 ).There were significantly differences between bladder cancer with different histological grades ( LTβR:18.2(2.1-31.3) × 10-3/ 28.4(16.6-36.2) × 10-3/47.9(34.3 -70.5) ×10-3,x2K-W=20.378;P65:4.9(1.3 - 12.0) × 10-3/7.4(3.0-21.9) × 10-3/17.0(10.0 ～28.3)× 10-3 ,x2K-W2 =15.219,P all ＜0.05) and lymph node metastasis (LTβR:27.2(9.7-40.1) ×10-3/39.4(26.7 -52.6) ×10-3,Z=-2.552; P65:7.4(2.3-15.6) ×10-3/13.4(6.7-23.3) ×10-3,Z=-2.026,P＜0.05).(2)The positive rates of LTβR and phosphorylated P65 ( p-P65 ) protein in cancer were higber than those of normal group (LTβR:69.8％/13.3％,x2 =16.600 ; p-P65:56.2％/6.7％,x2 =12.220,P ＜ 0.05 ).Upregulation of LTβR and p-P65 were associated with the histological grade (LTβR:56.3％/70.0％/90.4％,x2 =7.055; p-P65:40.6％ /60.0％/76.2％,x2 =6.679,P ＜0.05) and with lymph node metastasis (LTβR:58.3％/92.0％,x2 =8.849; p-P65:52.1％/64.0％,x2 =5.088,P ＜0.05).(3)There was a positive correlation between LTβR and P65 expression ( mRNA:r =0.654,P ＜ 0.05,protein:r =0.399,P ＜ 0.05 )in the bladder cancer and cystitis (r =0.521,P＜0.05).Conclusions The activation of LTβR and P65 was associated with progression and metastasis of bladder cancer.The activation of classical NF-κB pathway by LTβR may be achieved by P65.
