CAJ | 학술논문

目的建立大鼠慢性前脑缺血基础上的脑过度灌注模型.方法选取雄性Wistar大鼠72只依随机数字表均分为两个模型建造组.缺血模型采用双侧颈总动脉结扎,随机数字表分为空白对照、假手术组、缺血2周组、缺血4周组,每组9只,结扎前后分别测定脑额叶血流、比较各组行为学评分、脑梗死面积.过度灌注模型是在缺血模型基础上,再灌注同时经尾静脉持续输注去氧肾上腺素4μg·kg-1·min-1,使再灌注后脑额叶血流超过基础值的200%.随机分为空白对照组、盐水组、过度灌注0.5 h组、过度灌注2 h组,每组9只,再灌注前后分别测定脑额叶血流,比较各组行为学评分、血脑屏障通透性、脑干湿重比值.结果大鼠双侧颈动脉结扎后前脑血流减少可达67%±2%,脑过度灌注组与盐水输注组的脑血流变化值差异有统计学意义(P＜0.01).缺血2周组的神经功能评分、脑梗死面积与正常对照组差异无统计学意义,缺血4周组的脑梗死面积与正常对照组差异有统计学意义.脑过度灌注2 h组的血脑屏障通透性改变有统计学意义(P＜0.05).结论结扎大鼠双侧颈总动脉2周后脑过度灌注2 h可较好地建立大鼠脑过度灌注综合征模型.
목적 건립대서만성전뇌결혈기출상적뇌과도관주모형.방법 선취웅성Wistar대서72지의수궤수자표균분위량개모형건조조.결혈모형채용쌍측경총동맥결찰,수궤수자표분위공백대조、가수술조、결혈2주조、결혈4주조,매조9지,결찰전후분별측정뇌액협혈류、비교각조행위학평분、뇌경사면적.과도관주모형시재결혈모형기출상,재관주동시경미정맥지속수주거양신상선소4μg·kg-1·min-1,사재관주후뇌액협혈류초과기출치적200%.수궤분위공백대조조、염수조、과도관주0.5 h조、과도관주2 h조,매조9지,재관주전후분별측정뇌액협혈류,비교각조행위학평분、혈뇌병장통투성、뇌간습중비치.결과 대서쌍측경동맥결찰후전뇌혈류감소가체67%±2%,뇌과도관주조여염수수주조적뇌혈류변화치차이유통계학의의(P＜0.01).결혈2주조적신경공능평분、뇌경사면적여정상대조조차이무통계학의의,결혈4주조적뇌경사면적여정상대조조차이유통계학의의.뇌과도관주2 h조적혈뇌병장통투성개변유통계학의의(P＜0.05).결론 결찰대서쌍측경총동맥2주후뇌과도관주2 h가교호지건립대서뇌과도관주종합정모형.
Objective To establish the cerebral hyper-perfusion model after chronic forebrain ischemia in rats. Methods A total of 72 male rats were equally randomized into 2 modeling groups. The ligation of bilateral common carotid artery could induce chronic forebrain ischemia. And 36 rats were randomly grouped by ischemia duration: control group ( n = 9 ), sham group ( n = 9 ), 2-wcek ischemia group ( n = 9 ) and 4-wcek ischemia group ( n = 9 ). The blood flow in frontal lobe was measured at pre- and post-ligation. The neurological score and cerebral infarction area were also compared among the groups. The min-1 via tail vein to produce cerebrally hyperperfused blood flow rate over 200% of baseline following chronic ischemia. According to cerebral hyper-perfusion duration, 36 rats were randomly assigned into 4 groups: control group ( n = 9 ), saline infusion group ( n = 9 ), 30-minute cerebral hyper-perfusion group ( n = 9) and 2-hour cerebral hyper-perfusion group ( n = 9). The blood flow in frontal lobe was measured before and after cerebral hyper-perfusion. The neurological score, blood-brain barrier permeability and drywet weight ratio of brain also were compared among the groups. Results The forebrain blood flow decreased by 67% ± 2% after the ligation of bilateral common carotid artery. There was significant difference between cerebral hyper-perfusion and saline infusion groups ( P ＜ 0. 01 ). No statistic difference was observed in neurological score and cerebral infarction area between 2-week ischemia and control groups. But it was obvious between 4-week ischemia and control groups. The permeability in blood-brain barrier of rats significantly increased in 2-hour hyper-perfusion group ( P ＜ 0.05 ). Conclusion The 2-hour duration of cerebral hyper-perfusion following a 2-week ligation of bilateral common carotid artery may establish a reliable cerebral hyper-perfusion model in rats.