CAJ | 학술논문

目的观察电针对慢性脑缺血大鼠学习记忆功能和海马神经组织形态学的影响。方法雄性Sprague-Dawley( SD)大鼠120只，采用改良永久性结扎双侧颈总动脉法造成慢性脑缺血模型，将造模成功的104只分为模型组和电针组，每组52只，每组再分为术后第1，2，4，6周4个时间点，每个时间点13只。电针组采用电针治疗；利用Morris水迷宫、HE染色分别在术后第1，2，4，6周观察2组空间学习能力和记忆能力的变化情况及海马神经组织形态学变化。结果电针组大鼠在术后第2，4，6周时学习记忆能力明显高于同时间点模型组大鼠(P＜0.05或P＜0.01)；电针组大鼠海马齿状回区神经细胞结构紧密，排列整齐，层次丰富。结论电针能够改善慢性脑缺血大鼠空间学习能力和记忆能力，并能改善慢性脑缺血大鼠受损海马组织形态学。
목적 관찰전침대만성뇌결혈대서학습기억공능화해마신경조직형태학적영향。방법 웅성Sprague-Dawley( SD)대서120지，채용개량영구성결찰쌍측경총동맥법조성만성뇌결혈모형，장조모성공적104지분위모형조화전침조，매조52지，매조재분위술후제1，2，4，6주4개시간점，매개시간점13지。전침조채용전침치료；이용Morris수미궁、HE염색분별재술후제1，2，4，6주관찰2조공간학습능력화기억능력적변화정황급해마신경조직형태학변화。결과 전침조대서재술후제2，4，6주시학습기억능력명현고우동시간점모형조대서(P＜0.05혹P＜0.01)；전침조대서해마치상회구신경세포결구긴밀，배렬정제，층차봉부。결론 전침능구개선만성뇌결혈대서공간학습능력화기억능력，병능개선만성뇌결혈대서수손해마조직형태학。
Objective To observe the effects of electro-acupuncture ( EA ) on learning, memory and the morphology of hippocampal neural tissues in rats with a model of chronic cerebral ischemia.Methods One hundred and twenty male Sprague-Dawley rats were used. Chronic cerebral ischemia models were successfully established in 104 of them, and those rats were randomly divided into an EA group and a model group with 52 rats each. These were further subdivided into 1,2, 4 and 6 week subgroups with 13 rats in each. The EA group was given EA. The changes in spatial learning and memory ability were observed using a Morris water maze. The morphological changes in hippocampal nerve tissue were observed by HE staining.Results The escape latency in the EA group was significantly different from the model group at the 2nd, 4th and 6th week. The nerve cells in the dentate gyrus were more tightly and consistently lined-up and had rich layers, and the structures in the EA group were better than in the model group.Conclusions EA can improve spatial learning and memory and promote the repair of injury after cerebral ischemia.