中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
4期
296-298
,共3页
杨玉英%郎振为%兰孟东%石晓红%马沛卿%沈冰%王培之%徐道振
楊玉英%郎振為%蘭孟東%石曉紅%馬沛卿%瀋冰%王培之%徐道振
양옥영%랑진위%란맹동%석효홍%마패경%침빙%왕배지%서도진
CD4淋巴细胞计数%CD25淋巴细胞计数%T淋巴细胞%肝炎,乙型,慢性%免疫耐受
CD4淋巴細胞計數%CD25淋巴細胞計數%T淋巴細胞%肝炎,乙型,慢性%免疫耐受
CD4림파세포계수%CD25림파세포계수%T림파세포%간염,을형,만성%면역내수
CD4 lymphocyte count%CD25 lymphocyte count%T-lymphocytes%Hepatitis B,chronic%Immune tolerance
目的 观察慢性HBV感染患者免疫耐受期与免疫清除期肝组织中CD4+ CD25+调节性T细胞的表达及分布情况.方法 应用免疫组织化学法检测19例免疫耐受期及12例免疫清除期慢性乙型肝炎患者肝组织中FoxP3的表达,6例正常肝组织为对照.结果 FoxP3阳性信号位于淋巴细胞胞核内,阳性细胞主要聚集在汇管区,肝窦内亦可见散在单个淋巴细胞呈阳性.在免疫耐受期及免疫清除期患者肝组织中FoxP3较正常肝组织明显增加(P<0.01),免疫清除期患者肝组织内Fox P3明显高于免疫耐受期患者(P<0.01).免疫清除期患者肝组织中FoxP3阳性标记指数与ALT、HBeAg及HBV-DNA水平无明显相关性.免疫耐受期与免疫清除期两组相比,在年龄、ALT、TBIL、PTA、HBeAg及HBV-DNA水平方面差异均有统计学意义.结论 肝组织中CD4+ CD25+调节性T细胞在慢性HBV感染慢性化和抑制免疫,控制肝脏炎症反应方面可能起了重要作用.
目的 觀察慢性HBV感染患者免疫耐受期與免疫清除期肝組織中CD4+ CD25+調節性T細胞的錶達及分佈情況.方法 應用免疫組織化學法檢測19例免疫耐受期及12例免疫清除期慢性乙型肝炎患者肝組織中FoxP3的錶達,6例正常肝組織為對照.結果 FoxP3暘性信號位于淋巴細胞胞覈內,暘性細胞主要聚集在彙管區,肝竇內亦可見散在單箇淋巴細胞呈暘性.在免疫耐受期及免疫清除期患者肝組織中FoxP3較正常肝組織明顯增加(P<0.01),免疫清除期患者肝組織內Fox P3明顯高于免疫耐受期患者(P<0.01).免疫清除期患者肝組織中FoxP3暘性標記指數與ALT、HBeAg及HBV-DNA水平無明顯相關性.免疫耐受期與免疫清除期兩組相比,在年齡、ALT、TBIL、PTA、HBeAg及HBV-DNA水平方麵差異均有統計學意義.結論 肝組織中CD4+ CD25+調節性T細胞在慢性HBV感染慢性化和抑製免疫,控製肝髒炎癥反應方麵可能起瞭重要作用.
목적 관찰만성HBV감염환자면역내수기여면역청제기간조직중CD4+ CD25+조절성T세포적표체급분포정황.방법 응용면역조직화학법검측19례면역내수기급12례면역청제기만성을형간염환자간조직중FoxP3적표체,6례정상간조직위대조.결과 FoxP3양성신호위우림파세포포핵내,양성세포주요취집재회관구,간두내역가견산재단개림파세포정양성.재면역내수기급면역청제기환자간조직중FoxP3교정상간조직명현증가(P<0.01),면역청제기환자간조직내Fox P3명현고우면역내수기환자(P<0.01).면역청제기환자간조직중FoxP3양성표기지수여ALT、HBeAg급HBV-DNA수평무명현상관성.면역내수기여면역청제기량조상비,재년령、ALT、TBIL、PTA、HBeAg급HBV-DNA수평방면차이균유통계학의의.결론 간조직중CD4+ CD25+조절성T세포재만성HBV감염만성화화억제면역,공제간장염증반응방면가능기료중요작용.
Objective To investigate the expression and distribution of intrahepatic CD4+ CD25+regulatory T cells in immuno-tolerant and immuno-elearanee phase of patients with chronic hepatitis B. Methods The expression of FoxP3 was detected in 19 cases of immuno-tolerant phase and 12 cases of immuno-clearance phase by immunohistochemistry. The relation between the intrahepatic expression of FoxP3 and the clinico-pathological features were analyzed. Results The positive signal of FoxP3 is located in nuclear of lymphocyte and mainly aggregated in portal areas as well as occasionally scattered in hepatic sinusoids. The expression of intrahepatic FoxP3 in the group of immuno-tolerant phase was significantly increased than those in normal control(P<0.01), and greatly decreased than those in immuno-clearance phase (P<0.01). No correlation was observed among the expression of intrahepatic FoxP3, ALT, levels of HBV DNA, HBeAg positive, in patients of immuno-clearanee phase, respectively. There were significant differences between immuuo-tolerant phase and immuno-clearance phase age, ALT, TBIL, PTA, HBV-DNA and detection of HBeAg but not in sex and family history of HBV infection. Conclusion CD4+ CD25+ regulatory T cells may play important roles in the clearance of HBV as well as in liver inflammation and injury during chronic HBV infection.
