国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2009年
1期
1-3
,共3页
饶海林%曾海鹏%张珂%袁涛%张雪梅
饒海林%曾海鵬%張珂%袁濤%張雪梅
요해림%증해붕%장가%원도%장설매
包涵体%rhIL-6%生物酶解法%超声破碎法
包涵體%rhIL-6%生物酶解法%超聲破碎法
포함체%rhIL-6%생물매해법%초성파쇄법
Inclusion body%rhIL-6%Enzyme digestion%Ultrasonication
目的 建立重组人白细胞介素-6(recombinant human interleukin-6,rhIL-6)包涵体精制工艺,获取高纯度的包涵体蛋白.方法 采用超声破碎法和生物酶解法破碎细胞;摸索洗涤缓冲液中尿素的适当浓度,经多次洗涤获取高纯度的包涵体蛋白.结果 成功建立了rhIL-6包涵体蛋白的精制工艺,其中尿素浓度为2.0 mol/L,溶菌酶浓度达到0.8 mg/g菌体.包涵体蛋白洗涤后纯度达到90%以上.结论 建立合理的精制工艺,包涵体蛋白纯度达到90%以上.
目的 建立重組人白細胞介素-6(recombinant human interleukin-6,rhIL-6)包涵體精製工藝,穫取高純度的包涵體蛋白.方法 採用超聲破碎法和生物酶解法破碎細胞;摸索洗滌緩遲液中尿素的適噹濃度,經多次洗滌穫取高純度的包涵體蛋白.結果 成功建立瞭rhIL-6包涵體蛋白的精製工藝,其中尿素濃度為2.0 mol/L,溶菌酶濃度達到0.8 mg/g菌體.包涵體蛋白洗滌後純度達到90%以上.結論 建立閤理的精製工藝,包涵體蛋白純度達到90%以上.
목적 건립중조인백세포개소-6(recombinant human interleukin-6,rhIL-6)포함체정제공예,획취고순도적포함체단백.방법 채용초성파쇄법화생물매해법파쇄세포;모색세조완충액중뇨소적괄당농도,경다차세조획취고순도적포함체단백.결과 성공건립료rhIL-6포함체단백적정제공예,기중뇨소농도위2.0 mol/L,용균매농도체도0.8 mg/g균체.포함체단백세조후순도체도90%이상.결론 건립합리적정제공예,포함체단백순도체도90%이상.
Objective To establish the purification process of recombinant human interleukin.6(rhIL6)inclusion body and obtain more purified inclusion body.Methods The bacterial cells were broken by the methods of uhrasonication and enzyme digestion,the appropriate concentration of the urea was found out,and more purified inclusion bodies were obtained by washing inclusion bodies more than once.Results The purification process of rhIL-6 inclusion body Was established.The concentration of the urea Was 2.0 mol/L and the concentration of lysozyme Was 0.8 mg/g bacteria.The purity of inclusion body obtained could come up to more than 90%.Conclusions The reasonable purification process is established,and the purity of rhIL-6 inclusion body can reach more than 90%.
