国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2009年
14期
841-845,封3
,共6页
孙德俊%高丽%杨敬平%李姝楠
孫德俊%高麗%楊敬平%李姝楠
손덕준%고려%양경평%리주남
养阴活血汤%肺间质纤维化%原位杂交%转化生长因子β1%结缔组织生长因子%干扰素β1a
養陰活血湯%肺間質纖維化%原位雜交%轉化生長因子β1%結締組織生長因子%榦擾素β1a
양음활혈탕%폐간질섬유화%원위잡교%전화생장인자β1%결체조직생장인자%간우소β1a
Yangyin Huoxue Decoction%Pulmonary interstitial fibrosis%Hybridization in situ%Transforming growth factor β1%Connective tissue growth factor%Interferon-β1a
目的 观察养阴活血汤对博莱霉素诱导的大鼠肺间质纤维化疗效以及对转化生长因子β1(transforming growth factor-β1,TGF-β1)和结缔组织生长因子(connective tissue growth factor,CTGF)mRNA表达的影响.方法 96只雌性Wistar大鼠,随机分为正常组、模型组、中药组和干扰素β1a(interferon-β1a,IFN-β1a)组.气管内注入博莱霉素A2建立大鼠肺间质纤维化模型.中药组自造模次日始,每只大鼠灌胃浓缩中药每天20 ml/kg,IFN-β1a组皮下注射重组人IFN-β1a 4.6μg/kg,每周3次.正常组和模型组等体积生理盐水灌胃.各组大鼠在实验周期第3天、第7天、第14天和第28天腹主动脉放血法处死,进行肺系数检测,HE和VG染色.原位杂交测定大鼠肺组织中TGF-β1和CTGF的mRNA表达情况.结果 养阴活血汤能明显降低肺系数,使肺组织的病理改变减轻,可以减弱大鼠肺组织和原代培养肺成纤维细胞中TGF-β1 mRNA和CTGF mRNA的表达强度.结论 养阴活血汤可以减少TGF-β1 mRNA和CTGF mRNA的表达量,减弱细胞因子对肺间质纤维化的促进作用,从而起到抑制肺间质纤维化的作用.
目的 觀察養陰活血湯對博萊黴素誘導的大鼠肺間質纖維化療效以及對轉化生長因子β1(transforming growth factor-β1,TGF-β1)和結締組織生長因子(connective tissue growth factor,CTGF)mRNA錶達的影響.方法 96隻雌性Wistar大鼠,隨機分為正常組、模型組、中藥組和榦擾素β1a(interferon-β1a,IFN-β1a)組.氣管內註入博萊黴素A2建立大鼠肺間質纖維化模型.中藥組自造模次日始,每隻大鼠灌胃濃縮中藥每天20 ml/kg,IFN-β1a組皮下註射重組人IFN-β1a 4.6μg/kg,每週3次.正常組和模型組等體積生理鹽水灌胃.各組大鼠在實驗週期第3天、第7天、第14天和第28天腹主動脈放血法處死,進行肺繫數檢測,HE和VG染色.原位雜交測定大鼠肺組織中TGF-β1和CTGF的mRNA錶達情況.結果 養陰活血湯能明顯降低肺繫數,使肺組織的病理改變減輕,可以減弱大鼠肺組織和原代培養肺成纖維細胞中TGF-β1 mRNA和CTGF mRNA的錶達彊度.結論 養陰活血湯可以減少TGF-β1 mRNA和CTGF mRNA的錶達量,減弱細胞因子對肺間質纖維化的促進作用,從而起到抑製肺間質纖維化的作用.
목적 관찰양음활혈탕대박래매소유도적대서폐간질섬유화료효이급대전화생장인자β1(transforming growth factor-β1,TGF-β1)화결체조직생장인자(connective tissue growth factor,CTGF)mRNA표체적영향.방법 96지자성Wistar대서,수궤분위정상조、모형조、중약조화간우소β1a(interferon-β1a,IFN-β1a)조.기관내주입박래매소A2건립대서폐간질섬유화모형.중약조자조모차일시,매지대서관위농축중약매천20 ml/kg,IFN-β1a조피하주사중조인IFN-β1a 4.6μg/kg,매주3차.정상조화모형조등체적생리염수관위.각조대서재실험주기제3천、제7천、제14천화제28천복주동맥방혈법처사,진행폐계수검측,HE화VG염색.원위잡교측정대서폐조직중TGF-β1화CTGF적mRNA표체정황.결과 양음활혈탕능명현강저폐계수,사폐조직적병리개변감경,가이감약대서폐조직화원대배양폐성섬유세포중TGF-β1 mRNA화CTGF mRNA적표체강도.결론 양음활혈탕가이감소TGF-β1 mRNA화CTGF mRNA적표체량,감약세포인자대폐간질섬유화적촉진작용,종이기도억제폐간질섬유화적작용.
Objective To explore the treatment effect of Yangyin Huoxue Decoctior on pulmonary interstitial fibrosis in early stage and the mRNA expression of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor(CTGF) in lung tissue and primary cultural fibroblasts. Methods Ninety-six female Wistar rats were randomly assigned into four groups:normal group, model group, traditional Chinese medicine(TCM) group and interferon-β1a(IFN-β1a) group. In each group, there were 24 rats. Models were founded by bleomycin A2. The rats in TCM group were treated with gastric perfusion of 20 ml/kg per day the decoction and IFN-β1a group were treated with IFN-β1a (4.6 μg/kg) through subcutaneous injection. The rats were killed at 3, 7, 14, 28 days of feeding, then the pulmonary coefficients, hematoxylin-eosin staining and Van Gieson Chan biter acid fuchinacid staining were done and the mRNA expressions of TGF-β1 and CTGF were detected by the nuclic acid by hybridization in situ technique. Results Yangyin Huoxue Decoction decreased pulmonary coefficients significantly. It also weakened the level of fibrosis development of lung and the mRNA expression of TGF-β1 and CTGF in lung tissue. Conclusions Yangyin Huoxue Decoction can decrease the mRNA expression of TGF-β1 and CTGF in lung tissue. The formation of pulmonary interstitial fibrosis affected by the cytokines is weakened, in order to inhibit fibrotic development.
