中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2008年
4期
233-235
,共3页
陈绍亮%许兰文%陈雪芬%顾宇参%陈曙光%李蓓蕾%刘文官%石洪成%乔伟伟%吴直江%高奇蓉
陳紹亮%許蘭文%陳雪芬%顧宇參%陳曙光%李蓓蕾%劉文官%石洪成%喬偉偉%吳直江%高奇蓉
진소량%허란문%진설분%고우삼%진서광%리배뢰%류문관%석홍성%교위위%오직강%고기용
杀伤细胞%细胞活素类%锝%同位素标记%药代动力学%动物,实验
殺傷細胞%細胞活素類%锝%同位素標記%藥代動力學%動物,實驗
살상세포%세포활소류%득%동위소표기%약대동역학%동물,실험
Killer cells%Cytokines%Technetium%Isotope labeling%Pharmacokinetice%Animals,laboratory
目的 观察细胞因子诱导杀伤(CIK)细胞在动物体内分布的特点.方法 99Tcm标记CIK细胞采用亚锡还原法.昆明种小白鼠40只,按随机数字表法分为8组,每组5只,进行小鼠体内分布实验,计算每克组织百分注射剂量率(%ID/g).新西兰家兔3只行动态显像,连续观察99Tcm-CIK细胞体内分布图像特点.结果 分离纯化后99Tcm-CIK细胞的放化纯＞95%,室温下6 h内保持稳定.小白鼠体内99Tcm-CIK细胞血液清除较快,观察到血液中1和24 h的再分布现象.99Tcm-CIK细胞主要分布于肺、肝、脾和肾,心脏、脑、骨骼和肌肉等其他脏器组织均分布较少.家兔动态显像示99Tcm-CIK细胞早期分布以肺为主,随时间延长肺内放射性逐渐减少,肝放射性逐渐增多,并超过肺部放射性,2 d后仍可见肝放射性浓聚.心脏、脑、骨骼和肌肉等其他脏器组织均未见明显放射性分布.结论 99Tcm-CIK细胞在血循环中滞留时间较短,注射后1 h内主要聚集于肺,随后肺的摄取逐渐减少,其主要浓聚于肝和脾,尤以肝为甚.
目的 觀察細胞因子誘導殺傷(CIK)細胞在動物體內分佈的特點.方法 99Tcm標記CIK細胞採用亞錫還原法.昆明種小白鼠40隻,按隨機數字錶法分為8組,每組5隻,進行小鼠體內分佈實驗,計算每剋組織百分註射劑量率(%ID/g).新西蘭傢兔3隻行動態顯像,連續觀察99Tcm-CIK細胞體內分佈圖像特點.結果 分離純化後99Tcm-CIK細胞的放化純＞95%,室溫下6 h內保持穩定.小白鼠體內99Tcm-CIK細胞血液清除較快,觀察到血液中1和24 h的再分佈現象.99Tcm-CIK細胞主要分佈于肺、肝、脾和腎,心髒、腦、骨骼和肌肉等其他髒器組織均分佈較少.傢兔動態顯像示99Tcm-CIK細胞早期分佈以肺為主,隨時間延長肺內放射性逐漸減少,肝放射性逐漸增多,併超過肺部放射性,2 d後仍可見肝放射性濃聚.心髒、腦、骨骼和肌肉等其他髒器組織均未見明顯放射性分佈.結論 99Tcm-CIK細胞在血循環中滯留時間較短,註射後1 h內主要聚集于肺,隨後肺的攝取逐漸減少,其主要濃聚于肝和脾,尤以肝為甚.
목적 관찰세포인자유도살상(CIK)세포재동물체내분포적특점.방법 99Tcm표기CIK세포채용아석환원법.곤명충소백서40지,안수궤수자표법분위8조,매조5지,진행소서체내분포실험,계산매극조직백분주사제량솔(%ID/g).신서란가토3지행동태현상,련속관찰99Tcm-CIK세포체내분포도상특점.결과 분리순화후99Tcm-CIK세포적방화순＞95%,실온하6 h내보지은정.소백서체내99Tcm-CIK세포혈액청제교쾌,관찰도혈액중1화24 h적재분포현상.99Tcm-CIK세포주요분포우폐、간、비화신,심장、뇌、골격화기육등기타장기조직균분포교소.가토동태현상시99Tcm-CIK세포조기분포이폐위주,수시간연장폐내방사성축점감소,간방사성축점증다,병초과폐부방사성,2 d후잉가견간방사성농취.심장、뇌、골격화기육등기타장기조직균미견명현방사성분포.결론 99Tcm-CIK세포재혈순배중체류시간교단,주사후1 h내주요취집우폐,수후폐적섭취축점감소,기주요농취우간화비,우이간위심.
Objective The aim of this research was to investigate the in vivo distribution of cytokine induced killer(CIK)cell after intravenous injection in animals.Methods CIK cell was labeled with 99Tcm.Forty mice were equally divided into 8 groups.These mice were killed at 3,15,30 min and 1,3,6,24,48 h after intravenous 99Tcm-CIK cell injection.The radioactivity of blood,heart,lung,liver,spleen,kidney,brain,bone and muscle were measured.Three New Zealand rabbits were scanned by dynamic SPECT imaging after intravenous injection of 37 MBq 99Tcm-CIK cell.Serial images over whole body were acquired from immediately after injection to 48 h later.Results The radiochemical purity of 99Tcm-CIK cell Was over 95% and remained stable within 6 h at room temperature.The clearance of 99Tcm-CIK cell in blood was fast.High uptake of 99Tcm-CIK cell Was noted in lung,liver,spleen and kidney.Little uptake was found in heart,brain,bone and muscle.Dynamic SPECT imaging showed that 99Tcm-CIK cell accumulated in lungs early after injection and cleared thereafter.Then the uptake in liver increased and remained there after 2 d.There Was little uptake in heart,brain,bone and muscle.Conclusions 99Tcm-CIK cell clears fast in the blood after iniection.Within 1 h,the tracer mainly accumulates in lungs.Then the uptake is mostly in liver and spleen.
