CAJ | 학술논문

目的通过建立高脂血症性急性胰腺炎大鼠模型,探讨高脂血症可能加重急性胰腺炎病程的机制.方法 D-果糖饮食诱导SD大鼠的产生高脂血症,在此基础上通过腹腔注射蛙皮素诱导急性胰腺炎,并且设立正常组、高脂血症组和急性胰腺炎组作为对照.8周后,取大鼠血浆检测血糖、血脂和游离脂肪酸等指标;收集大鼠胰腺以及胰周组织,检测组织匀浆中ADP与ATP比值,通过Western blot法检测组织中caspase-3和caspase-8等蛋白以及其底物的表达情况,对组织切片进行HE染色并通过TUNEL法分析组织凋亡和坏死的情况.结果高脂血症性急性胰腺炎大鼠的血浆中血脂、游离脂肪酸等指标偏高;caspase-3和caspase-8活性片断表达下调,而其底物蛋白降解较少;TUNEL分析阳性率偏低,以上提示胰腺组织凋亡蛋白活性受到一定的抑制;另外,ADP与ATP比值相对较高,提示胰腺组织更易产生坏死反应.结论笔者推测高脂血症可能通过促进大鼠胰腺组织由凋亡趋向坏死,从而加重急性胰腺炎病变程度.
목적 통과건립고지혈증성급성이선염대서모형,탐토고지혈증가능가중급성이선염병정적궤제.방법 D-과당음식유도SD대서적산생고지혈증,재차기출상통과복강주사와피소유도급성이선염,병차설립정상조、고지혈증조화급성이선염조작위대조.8주후,취대서혈장검측혈당、혈지화유리지방산등지표;수집대서이선이급이주조직,검측조직균장중ADP여ATP비치,통과Western blot법검측조직중caspase-3화caspase-8등단백이급기저물적표체정황,대조직절편진행HE염색병통과TUNEL법분석조직조망화배사적정황.결과 고지혈증성급성이선염대서적혈장중혈지、유리지방산등지표편고;caspase-3화caspase-8활성편단표체하조,이기저물단백강해교소;TUNEL분석양성솔편저,이상제시이선조직조망단백활성수도일정적억제;령외,ADP여ATP비치상대교고,제시이선조직경역산생배사반응.결론 필자추측고지혈증가능통과촉진대서이선조직유조망추향배사,종이가중급성이선염병변정도.
Objective To establish the model of hyperlipidemie acute pancreatitis(AP)to explore the mechanism of hyperlipidemia in aggravating AP.Methods Sprague-Dawley rats were divided into four groups at radmon:1)control group(C);2)hyperlipidemic group(TG);3)acute pancreatitis group(AP);4)hyperlipidemic acute pancreatitis group(HAP).After 8 weeks,blood,pancreas and peripancreas tissue were collected from each rats for determination of various parameters.Results The levels of hyperilidemic index were elevated in HAP group compared with those of other groups.The ADP/ATP ratio was obviously increased in HAP group.Caspase-3 and caspase-8 of HAP group presented in zymogen forms,while in AP group they were in activity forms.After the pancreas issue were stained bv HE and further analyzed through TUNEL assay,we found that there were more pancreatic acinar cells undergoing necrosis in HAP group.Conclusion Hyperilidemia may promote the course of apoptosis to necrosis of pancreatic acinar cells,which may be the mechanism of deterioration in hyperlipidemic acute pancreatitis.