CAJ | 학술논문

目的观察氧化应激、转化生长因子-β1和巢蛋白表达变化在甲状腺功能亢进大鼠肾损害中的作用.结果优甲乐灌胃法制作甲亢大鼠模型,测定25,45,60 d大鼠肾组织中抗氧化酶:超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性,丙二醛(MDA)含量及TGF-β1、Nestin表达变化.结果随甲亢病程延长,CAT、GSH-Px、SOD活性逐渐降低,MDA含量逐渐增加;TGF-β1表达逐渐增多,而Nestin表达逐渐减少;且TGF-β1及Nestin表达均与氧化应激有相关性.结论氧化应激、TGF-β1表达增加及足细胞损伤相互作用共同参与甲亢肾脏损伤过程.
목적 관찰양화응격、전화생장인자-β1화소단백표체변화재갑상선공능항진대서신손해중적작용.결과 우갑악관위법제작갑항대서모형,측정25,45,60 d대서신조직중항양화매:초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)、과양화경매(CAT)활성,병이철(MDA)함량급TGF-β1、Nestin표체변화.결과 수갑항병정연장,CAT、GSH-Px、SOD활성축점강저,MDA함량축점증가;TGF-β1표체축점증다,이Nestin표체축점감소;차TGF-β1급Nestin표체균여양화응격유상관성.결론 양화응격、TGF-β1표체증가급족세포손상상호작용공동삼여갑항신장손상과정.
Objective To investigate effects of oxidative stress, expression change of transforming growth factor-β1 and Nestin in re-nal injury of hyperthyroidism rats. Methods The model of hyperthyroidism rat was made by giving them levothyroxine (L-T4). The activity of SOD, CAT, GSH-Px and the level of MDA on the 25th day, the 45th day, and the 60th day were determined by chromatometry in renal cor-tex.. The expressions of TGF-β1 and Nestin on the 25th day, the 45th day, and the 60th day were determined by immunohistochemistry. Results Along with the course of hyperthyroidism, it showed activity of SOD, CAT, GSH-Px and the expression of Nestin reduced, while the level of MDA and the expression of TGF-β1 increased. Conclusion Oxidative stress can increas the expression of TGF-β1 and podocyte in-jury may played an important role in hyperthyroidism renal injury.